Devi Naorem Bela, Pakshirajan Kannan
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, India.
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, India.
Bioresour Technol. 2025 Jul;427:132401. doi: 10.1016/j.biortech.2025.132401. Epub 2025 Mar 14.
This study explores the impact of syngas, a mixture of CO (carbon monoxide), CO (carbon dioxide) and H (hydrogen), composition on production of ethanol and other metabolites by using various Clostridia. Clostridium carboxidivorans, Clostridium ljungdahlii, and Clostridium ragsdalei were examined to convert CO-rich syngas into ethanol and other valuable products, and C. carboxidivorans was shown to produce maximum ethanol at a high initial CO concentration (80 % CO: 10 % CO: 10 % H). In addition, other C2-C6 compounds, viz. lactate, propionate, butyrate, 2,3-butanediol, butanol, isovalerate, hexanol, were produced by C. carboxidivorans, C. ljungdahlii, and C. ragsdalei, indicating diversified product formation through the Wood-Ljungdahl pathway. Modified Gompertz and Logistic models were successfully applied to describe the kinetics of cell growth and ethanol production by Clostridia via syngas fermentation. The findings emphasize optimization of syngas composition for maximum production of ethanol and other valuable biochemicals, providing a sustainable approach to biofuels and bioproduct production.
本研究通过使用各种梭菌，探讨了合成气（一氧化碳、二氧化碳和氢气的混合物）组成对乙醇及其他代谢产物生成的影响。研究了羧基还原梭菌、Ljungdahlii梭菌和Ragsdalei梭菌将富含一氧化碳的合成气转化为乙醇及其他有价值产物的能力，结果表明羧基还原梭菌在高初始一氧化碳浓度（80%一氧化碳：10%二氧化碳：10%氢气）下产生的乙醇最多。此外，羧基还原梭菌、Ljungdahlii梭菌和Ragsdalei梭菌还产生了其他C2 - C6化合物，即乳酸、丙酸、丁酸、2,3 - 丁二醇、丁醇、异戊酸、己醇，这表明通过伍德 - 隆达尔途径形成了多样化的产物。修正的戈姆珀茨模型和逻辑斯蒂模型成功地用于描述梭菌通过合成气发酵进行细胞生长和乙醇生产的动力学。这些发现强调了优化合成气组成以实现乙醇及其他有价值生物化学物质的最大产量，为生物燃料和生物产品生产提供了一种可持续的方法。
