Bahrami Gholamreza, Sajadimajd Soraya, Kazemi Farnoush, Yarmohammadi Fatemeh, Miraghaee Seyed Shahram
Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran.
Naunyn Schmiedebergs Arch Pharmacol. 2025 Mar 17. doi: 10.1007/s00210-025-04027-9.
Metabolic dysfunction-associated steatotic liver disease (MASLD) is designated as the main hepatic evidence of metabolic syndrome. Over the past few decades, the use of herbal medications in the treatment of MASLD has been increasingly studied due to evidence of their potential therapeutic mechanisms, wide availability, and lower side effects. This study aimed to scrutinize the effect of an oligosaccharide isolated from Rosa canina on high-cholesterol diet-induced MASLD rabbits. Twenty-four New Zealand rabbits were categorized into three groups (eight rabbits in each group). The first group received only the standard diet. Others received 2% cholesterol for 120 days. Then, the rabbits were treated with 20 mg/kg of an isolated oligosaccharide daily by gavage for 60 days or 6 mg/kg of simvastatin as a standard. After 14-16 h of starvation, blood samples were collected to measure lipid profile and liver enzymes. In addition, histological sampling of the liver and thoracic aorta was done. Cholesterol and triglyceride were significantly decreased in the oligosaccharide-treated group (p < 0.05). Also, the activity of alanine aminotransferase, aspartate aminotransferase, and creatine phosphokinase decreased significantly. Lactate dehydrogenase activity was also decreased. Based on histopathological studies, the treatment with an isolated oligosaccharide prevented atherosclerotic changes and decreased liver injury. Our data suggest that an oligosaccharide isolated from Rosa canina possesses hypolipidemic and hepatoprotective activities which will be beneficial in MASLD.
代谢功能障碍相关脂肪性肝病（MASLD）被认定为代谢综合征的主要肝脏表现。在过去几十年中，由于草药具有潜在治疗机制的证据、广泛可得性以及较低的副作用，其在MASLD治疗中的应用受到越来越多的研究。本研究旨在探讨从犬蔷薇中分离出的一种寡糖对高胆固醇饮食诱导的MASLD兔的影响。将24只新西兰兔分为三组（每组8只）。第一组仅给予标准饮食。其他组给予2%胆固醇，持续120天。然后，将兔子每天经口灌胃给予20 mg/kg分离出的寡糖，持续60天，或给予6 mg/kg辛伐他汀作为对照。饥饿14 - 16小时后，采集血样以测量血脂谱和肝酶。此外，对肝脏和胸主动脉进行组织学采样。寡糖治疗组的胆固醇和甘油三酯显著降低（p < 0.05）。此外，丙氨酸氨基转移酶、天冬氨酸氨基转移酶和肌酸磷酸激酶的活性显著降低。乳酸脱氢酶活性也降低。基于组织病理学研究，分离出的寡糖治疗可预防动脉粥样硬化改变并减轻肝损伤。我们的数据表明，从犬蔷薇中分离出的寡糖具有降血脂和肝脏保护活性，这对MASLD有益。
