Kang Ya-Shuai, Zhu Hao-Wen, Zhao Xue, Ma Hao, Yang Yu, Xu Rui, Luo Fu-Yao, Li Er-Wei, Luo Qian, Zhang You-Zhi, Li Chang-Wei
School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing, China.
Beijing Institute of Pharmacology and Toxicology, Beijing, China.
Chem Biodivers. 2025 Aug;22(8):e202500701. doi: 10.1002/cbdv.202500701. Epub 2025 Apr 3.
A total of 46 endophytic fungi were isolated from Huperzia serrata and then cultured in a standard liquid medium and PDB medium. Cytotoxicity and antimicrobial activity assays revealed that seven crude extracts exhibited significant inhibitory effects on MCF-7 and SH-SY5Y cell lines; four crude extracts effectively inhibited the growth of Candida albicans, and 12 crude extracts demonstrated potent inhibition effects on Staphylococcus aureus. Strains displaying antimicrobial activity or potential for producing novel metabolites were selected for identification. In total, 14 strains representing 7 genera were identified, 5 of which had not been previously documented regarding their secondary metabolites. A fungus, Penicillium panissanguineum SZSS 4-2-2 was preliminarily selected for further chemical investigation. From the fermentation extract of P. panissanguineum SZSS 4-2-2, one new compound (1), one new natural product (2), and two known compounds (3 and 4) were isolated. The structures of these compounds were elucidated through spectral analysis and calculated nuclear magnetic resonance and electronic circular dichroism data. Compound 1 demonstrated a significantly stronger inhibitory effect on lipopolysaccharide-induced nitric oxide (NO) production in RAW 264.7 macrophages, with an IC value of 64.91 µM. By contrast, compounds 2, 3, and 4 exhibited only moderate inhibitory effects on NO production.
从蛇足石杉中总共分离出46株内生真菌，然后在标准液体培养基和马铃薯葡萄糖肉汤（PDB）培养基中培养。细胞毒性和抗菌活性测定表明，七种粗提物对MCF - 7和SH - SY5Y细胞系表现出显著的抑制作用；四种粗提物有效抑制白色念珠菌的生长，12种粗提物对金黄色葡萄球菌显示出强效抑制作用。选择具有抗菌活性或具有产生新代谢物潜力的菌株进行鉴定。总共鉴定出代表7个属的14株菌株，其中5株的次生代谢物以前未见报道。初步选择一株真菌，即血红青霉SZSS 4 - 2 - 2进行进一步的化学研究。从血红青霉SZSS 4 - 2 - 2的发酵提取物中，分离出一种新化合物（1）、一种新天然产物（2）和两种已知化合物（3和4）。通过光谱分析以及计算得到的核磁共振和电子圆二色性数据阐明了这些化合物的结构。化合物1对脂多糖诱导的RAW 264.7巨噬细胞中一氧化氮（NO）的产生表现出显著更强的抑制作用，IC值为64.91 μM。相比之下，化合物2、3和4对NO的产生仅表现出中等程度的抑制作用。
