Alqaraleh Salameh, Al-Omari Laila, Mehyar Ghadeer, Alqaraleh Moath, Alshaer Walhan, Abdelnabi Hiba, Jaradat Sarah
Department of Nutrition and Food Science, The University of Jordan, Amman, Jordan.
Department of Medical Laboratory Sciences, Al-Ahliyya Amman University, Amman Jordan.
PLoS One. 2025 Mar 18;20(3):e0315771. doi: 10.1371/journal.pone.0315771. eCollection 2025.
Coumarin is a natural bioactive compound found in many plants and acquires antibacterial, antioxidant and anticoagulant activities. The antibacterial activity of coumarin has never been tested after being encapsulated in liposomes. This study was carried out to screen the main functional components of Rubus canescens DC crude extract (CE), develop a coumarin nanoliposome, and test its anti-bacterial and antioxidant properties.
R. canescens DC CE was screened for its main functional compounds using liquid chromatography-mass spectrometry (LC-MS). Pure commercial coumarin was loaded into liposomes and characterized in terms of surface morphology, hydrodynamic diameter, zeta potential, and encapsulation efficiency (EE). The antioxidant activity of coumarin was evaluated against ascorbic acid. The antibacterial activity of both coumarin alone and liposome-encapsulated coumarin against Staphylococcus aureus, Salmonella typhimurium, and Pseudomonas aeruginosa inoculated in soft white cheese (SWC) was also evaluated.
The predominant natural constituent of R. canescens DC CE, was coumarin. Comparing the DPPH scavenging activity of coumarin to that of ascorbic acid, coumarin exhibited an insignificant effect (p ≥ 0.05). The minimum inhibitory concentration (MIC) values for coumarin against P. aeruginosa, E. coli, Staph. aureus, S. typhimurium, and L. monocytogenes were 2.5, 2.5, 2.5, 1.25, and 1.25 µg/ml, respectively. The minimum bactericidal concentration (MBC) values for coumarin against these microorganisms were 5, 5, 5, 2.5, and 2.5 µg/ml, respectively. Coumarin was successfully loaded into nanoliposomes, which had a polydispersity index (PDI) of 0.36 ± 0.35 Đ, hydrodynamic diameter of 127.8 ± 0.3 nm, zeta-potential of -61.03 ± 2.9 mV, and EE of 40.93 ± 0.2%. Both the coumarin alone and the liposome loaded with coumarin showed antibacterial effects against the inoculated bacterial strains in SWC over a 30-day storage period at 4°C.
Coumarin was successfully formulated into a nanoliposome, and showed antibacterial activity against P. aeruginosa, S. aureus, and S. typhimurium.
香豆素是一种存在于许多植物中的天然生物活性化合物，具有抗菌、抗氧化和抗凝血活性。香豆素包裹于脂质体后的抗菌活性尚未得到测试。本研究旨在筛选灰毛泡粗提物（CE）的主要功能成分，制备香豆素纳米脂质体，并测试其抗菌和抗氧化性能。
采用液相色谱 - 质谱联用（LC - MS）技术筛选灰毛泡CE中的主要功能化合物。将纯的市售香豆素载入脂质体，并对其表面形态、水合直径、zeta电位和包封率（EE）进行表征。以抗坏血酸为对照评估香豆素的抗氧化活性。还评估了单独的香豆素以及脂质体包裹的香豆素对接种于软白奶酪（SWC）中的金黄色葡萄球菌、鼠伤寒沙门氏菌和铜绿假单胞菌的抗菌活性。
灰毛泡CE的主要天然成分是香豆素。将香豆素的DPPH清除活性与抗坏血酸的进行比较，香豆素表现出不显著的效果（p≥0.05）。香豆素对铜绿假单胞菌、大肠杆菌、金黄色葡萄球菌、鼠伤寒沙门氏菌和单核细胞增生李斯特菌的最低抑菌浓度（MIC）值分别为2.5、2.5、2.5、1.25和1.25μg/ml。香豆素对这些微生物的最低杀菌浓度（MBC）值分别为5、5、5、2.5和2.5μg/ml。香豆素成功载入纳米脂质体，其多分散指数（PDI）为0.36±0.35 Đ，水合直径为127.8±0.3 nm，zeta电位为 - 61.03±2.9 mV，包封率为40.93±0.2%。在4°C下储存30天期间，单独的香豆素和负载香豆素的脂质体均对SWC中接种的细菌菌株显示出抗菌作用。
香豆素成功制备成纳米脂质体，并对铜绿假单胞菌、金黄色葡萄球菌和鼠伤寒沙门氏菌显示出抗菌活性。
