Zheng Xiaoyan, Fu Zeshi, Qu Haowen, Lu Hongliang, Jiang Nanyue, Liu Ning, Li Meng, Wang Zhongjiang
College of Food Science, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030,China.
College of Food Science, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030,China.
Int J Biol Macromol. 2025 May;307(Pt 3):142151. doi: 10.1016/j.ijbiomac.2025.142151. Epub 2025 Mar 16.
Oxidative stress is an important cause of exercise fatigue formation. Nutritional intervention is an important way to modulate exercise fatigue. Lactoferrin (LF) and soybean protein (SP) are potential antioxidant bioactive components. Our findings demonstrate that SP-LF hybrid hydrolysates had effective 2,2-diphenylpicrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical scavenging activity and iron ion reducing ability. The synergistic effect between these hybrid hydrolysates were found to be superior to the single hydrolysate in terms of antioxidant level by the joint index analysis. These hybrid hydrolysates are characterized by high levels of amino acids with potential anti-fatigue effect: tyrosine (Tyr), phenylalanine (Phe), hydrophobic amino acid (HAAs) and branched-chain amino acids (BCAAs). In murine models, hybrid hydrolysates significantly prolonged weight-bearing swimming time, increased muscle/liver glycogen levels, decreased lactate, urea nitrogen, and malondialdehyde levels, and increased glutathione peroxidase, superoxide dismutase, catalase and ATPase activities. Pearson's correlation analysis established significant associations between antioxidant capacity and anti-fatigue efficacy. It alleviated fatigue through activating the Keap1/Nrf2/HO-1 signaling pathway, while increasing the expression levels of PGC-1α. These results collectively suggest that SP-LF hybrid hydrolysates demonstrate significant synergistic antioxidant and anti-fatigue activity and could be incorporated into functional foods as a dietary supplement to reduce fatigue.
氧化应激是运动性疲劳形成的重要原因。营养干预是调节运动性疲劳的重要途径。乳铁蛋白（LF）和大豆蛋白（SP）是潜在的抗氧化生物活性成分。我们的研究结果表明，SP-LF复合水解产物具有有效的2,2-二苯基苦味酰基自由基（DPPH）和2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除活性以及铁离子还原能力。通过联合指数分析发现，这些复合水解产物之间的协同效应在抗氧化水平方面优于单一水解产物。这些复合水解产物的特点是含有高水平的具有潜在抗疲劳作用的氨基酸：酪氨酸（Tyr）、苯丙氨酸（Phe）、疏水氨基酸（HAAs）和支链氨基酸（BCAAs）。在小鼠模型中，复合水解产物显著延长了负重游泳时间，提高了肌肉/肝脏糖原水平，降低了乳酸、尿素氮和丙二醛水平，并提高了谷胱甘肽过氧化物酶、超氧化物歧化酶、过氧化氢酶和ATP酶的活性。Pearson相关性分析确定了抗氧化能力与抗疲劳功效之间存在显著关联。它通过激活Keap1/Nrf2/HO-1信号通路来减轻疲劳，同时提高PGC-1α的表达水平。这些结果共同表明，SP-LF复合水解产物具有显著的协同抗氧化和抗疲劳活性，可作为膳食补充剂添加到功能性食品中以减轻疲劳。
