Taki T M, Nickerson P A
Lab Invest. 1985 Jul;53(1):91-100.
Changes in the adrenal cortex of the rat were studied by stereologic and autoradiographic techniques during the early phases of regeneration. The adrenal cortex was enucleated on the left side, the right kidney and adrenal gland were removed, and rats were given 1% sodium chloride as drinking solution according to the procedure for inducing adrenal regeneration hypertension. The growth of adrenocortical cells during early stages of regeneration can be divided into two phases. During the first stage (0 to day 3), the cells remaining attached to the capsule recovered from trauma and began to differentiate from zona glomerulosa-like to those characteristic of the zona fasciculata. Mitochondria also divided, and there was increased surface area per cell of total mitochondrial membranes. The number of cells decreased in this initial phase, although the average cell volume increased, in preparation for mitotic division. This conclusion is supported by an increase in the percentage of cells labeled with 3H-thymidine at day 3. The second period (end of day 3 to day 7) was a proliferative phase reflected in a large number of labeled cells and a maximal number of grains per volume (reflecting activity of 3H-thymidine incorporation) followed by a blunting of the wave of proliferation at day 7. As cells divided, the total mitochondrial membranes and cristae per cell decreased. The first week after enucleation is characterized first by preparation for cell division, and in the second phase of the first week a wave of proliferation occurs. At the end of the first week, fewer labeled cells were seen, and there was a decrease in cristae membranes.
采用体视学和放射自显影技术，对大鼠肾上腺皮质再生早期的变化进行了研究。在左侧肾上腺皮质摘除、右侧肾和肾上腺切除后，按照诱导肾上腺再生性高血压的程序，给大鼠饮用1%氯化钠溶液。肾上腺皮质细胞在再生早期的生长可分为两个阶段。在第一阶段（0至第3天），仍附着于被膜的细胞从创伤中恢复，并开始从类似球状带的细胞分化为束状带特征性的细胞。线粒体也进行了分裂，每个细胞的线粒体总膜表面积增加。尽管平均细胞体积增加，但在这个初始阶段细胞数量减少，为有丝分裂做准备。这一结论得到了第3天标记有3H-胸腺嘧啶核苷的细胞百分比增加的支持。第二个时期（第3天末至第7天）是一个增殖期，表现为大量标记细胞和每体积最大数量的银粒（反映3H-胸腺嘧啶核苷掺入活性），随后在第7天增殖波变钝。随着细胞分裂，每个细胞的线粒体总膜和嵴减少。摘除肾上腺后的第一周，首先以细胞分裂准备为特征，在第一周的第二阶段出现增殖波。在第一周结束时，可见标记细胞减少，嵴膜减少。
