Sarría R, Losada J, Bueno-López J L
Department of Neuroscience, School of Medicine and Dentistry, Basque Country University, Spain.
Histol Histopathol. 1995 Jul;10(3):603-9.
The proliferative activity, the organization and the corticosterone expression of adrenocortical cells in an experimental adrenal regeneration process after the transplantation of neonatal adrenal glands to adult hosts was investigated. Three days after transplantation, the medullar and the innermost adrenocortical cells of the neonatal adrenal glands showed degenerative and necrotic changes due to the lack of vascular supply. The remaining outermost adrenocortical cells did not display any PCNA immunoreaction. The first PCNA expression, pointing out the beginning of the proliferative cycle, was observed in a 45.4% of the adrenocortical cells, one week after transplantation. After three weeks, several regenerated adrenocortical nodules with a bigger size than the one observed in the previous periods were seen. In these nodules, while the outermost adrenocortical cells were disposed in parallel to the capsule or in rounded groups, the bulk of the regenerated mass width was composed of cells forming longitudinal cords. PCNA immunoreaction was almost exclusively restricted to subcapsular cells (62.5%) and to cells of the outermost portion of the cords (32.5%), the global percentage of PCNA immunopositive cells being 18.4%. Twelve weeks after transplantation, regenerated adrenocortical cells were arranged in three layers: glomerulosa, fasciculata and reticularis. Only 1.85% of the adrenocortical cells were PCNA immunopositive. Although in the early stages of the regeneration process, all the adrenocortical cells, both proliferating and non proliferating cells expressed corticosterone, a restriction of this immunoreactivity to the zonae fasciculata and reticularis was observed when cell zonation was apparent.
研究了将新生肾上腺移植到成年宿主后，实验性肾上腺再生过程中肾上腺皮质细胞的增殖活性、组织结构和皮质酮表达。移植后三天，新生肾上腺的髓质和最内层肾上腺皮质细胞因缺乏血管供应而出现退行性和坏死性变化。其余最外层肾上腺皮质细胞未显示任何增殖细胞核抗原（PCNA）免疫反应。移植一周后，在45.4%的肾上腺皮质细胞中观察到首次PCNA表达，表明增殖周期开始。三周后，可见几个再生的肾上腺皮质结节，其大小比之前各期观察到的结节更大。在这些结节中，最外层肾上腺皮质细胞与被膜平行排列或呈圆形聚集，而再生团块宽度的大部分由形成纵行索状的细胞组成。PCNA免疫反应几乎仅局限于被膜下细胞（62.5%）和索状结构最外层的细胞（32.5%），PCNA免疫阳性细胞的总体百分比为18.4%。移植十二周后，再生的肾上腺皮质细胞排列成三层：球状带、束状带和网状带。只有1.85%的肾上腺皮质细胞PCNA免疫阳性。尽管在再生过程的早期，所有肾上腺皮质细胞，无论是增殖细胞还是非增殖细胞都表达皮质酮，但当细胞分区明显时，这种免疫反应性局限于束状带和网状带。
