Korek Magdalena, Mehta Devang, Uhrig Glen R, Daszkowska-Golec Agata, Novak Ondrej, Buchcik Weronika, Marzec Marek
Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellonska 28, 40-032, Katowice, Poland.
Department of Biological Sciences, University of Alberta, 11455 Saskatchewan Drive, Edmonton, AB, T6G 2E9, Canada.
Sci Rep. 2025 Mar 18;15(1):9375. doi: 10.1038/s41598-025-94430-2.
In response to environmental changes, plants continuously make architectural changes in order to optimize their growth and development. The regulation of plant branching, influenced by environmental conditions and affecting hormone balance and gene expression, is crucial for agronomic purposes due to its direct correlation with yield. Strigolactones (SL), the youngest class of phytohormones, function to shape the architecture of plants by inhibiting axillary outgrowth. Barley plants harboring the mutation in the HvDWARF14 (HvD14) gene, which encodes the SL-specific receptor, produce almost twice as many tillers as wild-type (WT) Sebastian plants. Here, through hormone profiling and comparison of transcriptomic and proteomic changes between 2- and 4-week-old plants of WT and hvd14 genotypes, we elucidate a regulatory mechanism that might affect the tillering of SL-insensitive plants. The analysis showed statistically significant increased cytokinin content and decreased auxin and abscisic acid content in 'bushy' hvd14 compared to WT, which aligns with the commonly known actions of these hormones regarding branching regulation. Further, transcriptomic and proteomic analysis revealed a set of differentially expressed genes (DEG) and abundant proteins (DAP), among which 11.6% and 14.6% were associated with phytohormone-related processes, respectively. Bioinformatics analyses then identified a series of potential SL-dependent transcription factors (TF), which may control the differences observed in the hvd14 transcriptome and proteome. Comparison to available Arabidopsis thaliana data implicates a sub-selection of these TF as being involved in the transduction of SL signal in both monocotyledonous and dicotyledonous plants.
为响应环境变化，植物不断进行形态结构的改变以优化其生长发育。植物分枝的调控受环境条件影响，并影响激素平衡和基因表达，由于其与产量直接相关，因此对农艺学目的至关重要。独脚金内酯（SL）是最新一类植物激素，通过抑制侧枝生长来塑造植物的形态结构。携带HvDWARF14（HvD14）基因突变的大麦植株，该基因编码SL特异性受体，其分蘖数几乎是野生型（WT）塞巴斯蒂安植株的两倍。在这里，通过对WT和hvd14基因型2周龄和4周龄植株进行激素谱分析以及转录组和蛋白质组变化的比较，我们阐明了一种可能影响对SL不敏感植株分蘖的调控机制。分析表明，与WT相比，“丛生”的hvd14中细胞分裂素含量在统计学上显著增加，生长素和脱落酸含量降低，这与这些激素在分枝调控方面的已知作用一致。此外，转录组和蛋白质组分析揭示了一组差异表达基因（DEG）和丰度蛋白（DAP），其中分别有11.6%和14.6%与植物激素相关过程有关。生物信息学分析随后鉴定出一系列潜在的依赖SL的转录因子（TF），它们可能控制hvd14转录组和蛋白质组中观察到的差异。与现有的拟南芥数据比较表明，这些TF中的一部分参与了单子叶植物和双子叶植物中SL信号的转导。
