Kang Yuting, Li Qiujie, Ma Wanting, Xu Chao, Qiu Zhuoran, Jia Wei, Wang Pengtao
Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, 750004, Ningxia, China.
First Clinical Medical College, Ningxia Medical University, Yinchuan, 750004, Ningxia, China.
Ann Clin Microbiol Antimicrob. 2025 Mar 18;24(1):19. doi: 10.1186/s12941-025-00789-5.
This study aimed to assess the resistance phenotype, virulence phenotype, and genetic characteristics of a bla and bla co-harboring ST65 K2 Klebsiella pneumoniae (KP114), which was isolated from General hospital of Ningxia Medical University.
Antibiotic susceptibility test was determined by Vitek 2 Compact system. Multilocus Sequence typing (MLST), antimicrobial resistance and virulence genes were examined by PCR and Sanger sequencing. The virulence of KP114 was evaluated through string test, macrophage phagocytosis assay, serum resistance assay, and mouse infection model. Whole-genome sequencing was performed for further analysis of genetic information.
The presence of the bla and bla genes in KP114 confered resistance to multi-antibiotics. The hypervirulence of KP114 was demonstrated through various in vitro experiments and in vivo mouse infection model. KP114 was found to harbor two distinct plasmids: a drug-resistant plasmid (pKP114-NDM), classified as the IncX3 type, which contained various transfer elements including type IV coupling protein (T4CP) and type IV secretion system (T4SS), and a virulence plasmid (pKP114-vir) that exhibited a high sequence similarity with pLVPK. The results of the conjugation experiment showed that resistance and virulence traits were successfully transferred from KP114 to Escherichia coli EC600 and J53.
We reported a Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) strain of ST65 K2 serotype carrying the bla and bla, which exhibited hypervirulence and drug resistance with potential for transmission. This finding allows improved clinical surveillance and control of this clone, thereby holding considerable value for clinical treatment.
本研究旨在评估从宁夏医科大学总医院分离出的一株同时携带bla和bla的ST65 K2型肺炎克雷伯菌（KP114）的耐药表型、毒力表型及遗传特征。
采用Vitek 2 Compact系统进行药敏试验。通过PCR和Sanger测序检测多位点序列分型（MLST）、耐药基因和毒力基因。通过拉丝试验、巨噬细胞吞噬试验、血清耐药试验和小鼠感染模型评估KP114的毒力。进行全基因组测序以进一步分析遗传信息。
KP114中bla和bla基因的存在使其对多种抗生素耐药。通过各种体外实验和体内小鼠感染模型证明了KP114的高毒力。发现KP114携带两种不同的质粒：一种耐药质粒（pKP114-NDM），分类为IncX3型，包含各种转移元件，包括IV型偶联蛋白（T4CP）和IV型分泌系统（T4SS）；另一种毒力质粒（pKP114-vir），与pLVPK具有高度序列相似性。接合实验结果表明，耐药和毒力性状成功从KP114转移至大肠杆菌EC600和J53。
我们报道了一株携带bla和bla的ST65 K2血清型耐碳青霉烯类高毒力肺炎克雷伯菌（CR-hvKP）菌株，该菌株表现出高毒力和耐药性且具有传播潜力。这一发现有助于加强对该克隆的临床监测和控制，对临床治疗具有重要价值。
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