Wang Qian, Wang Hanyue, Wang Xuyang, Yang Cheng, Li Yongming, Liao Lanjie, Zhu Zuoyan, Wang Yaping, He Libo
State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
iScience. 2025 Feb 15;28(3):112033. doi: 10.1016/j.isci.2025.112033. eCollection 2025 Mar 21.
Grass carp reovirus (GCRV) causes hemorrhagic disease in grass carp, leading to significant economic losses in China's aquaculture. However, the cellular receptors responsible for the initiation of GCRV infection remain unclear. This study reveals that cell surface heparan sulfate (HS) acts as a crucial attachment receptor for GCRV. Removing HS with heparinase significantly reduces GCRV attachment and infection. Both HS and its homologue, heparin, inhibit the attachment of GCRV to cells. Altering HS levels in cells affects GCRV attachment and infection accordingly. GCRV outer capsid proteins VP5, VP56, and VP35, as well as purified GCRV virions, directly bind to HS. Pretreating GCRV with heparin or feeding grass carp with feed containing heparin significantly reduces mortality caused by GCRV infection. Collectively, these results highlight the crucial role of HS as an attachment receptor for GCRV and therefore provide a promising target for the prevention and control of this virus.
草鱼呼肠孤病毒（GCRV）可引发草鱼出血性疾病，给中国水产养殖业造成重大经济损失。然而，负责启动GCRV感染的细胞受体仍不清楚。本研究表明，细胞表面硫酸乙酰肝素（HS）是GCRV的关键附着受体。用肝素酶去除HS可显著降低GCRV的附着和感染。HS及其同系物肝素均可抑制GCRV与细胞的附着。改变细胞内HS水平会相应地影响GCRV的附着和感染。GCRV外 capsid蛋白VP5、VP56和VP35以及纯化的GCRV病毒粒子可直接与HS结合。用肝素预处理GCRV或用含肝素的饲料喂养草鱼可显著降低GCRV感染导致的死亡率。综上所述，这些结果突出了HS作为GCRV附着受体的关键作用，因此为该病毒的防控提供了一个有前景的靶点。
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