Özçelikay Akyildiz Göksu, Ünal Mehmet Altay, Gülden Seçil, Özkan Sibel A
Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Ankara, Turkiye.
Stem Cell Institute, Ankara University, Ankara, Turkiye.
Turk J Chem. 2024 Dec 16;49(1):79-88. doi: 10.55730/1300-0527.3712. eCollection 2025.
Sex-Determining Region Y-box 2 (SOX2) is a transcription factor protein. SOX2 expression is related to lymph node metastasis and distant metastasis in colorectal carcinomas. SOX2 was determined with the first magnetic disposable immunoplatform. The designed sandwich-shaped immune complexes were formed by a capture antibody, SOX2 protein, and biotinylated secondary antibodies (dAb/HRP). The sandwich-shaped immune complex was linked to carboxylic acid functionalized magnetic beads (HOOC-MBs). This magnetic bioconjugate was dropped on the surface of the screen-printed carbon electrode (SPCE). The amperometric measurement was performed at -0.20 V in the presence of hydroquinone (HQ) and HO against a silver pseudo-reference electrode. The optimization parameters affecting the immunoassay response were evaluated. The analytical evaluation of the magnetic disposable immunoplatform for the amperometric detection of SOX2 standards was done. The developed immunosensor shows high sensitivity (LOD of 1.37 ng mL) and a short analysis time (15 min). Potential interfering compounds found in serum samples were tested. The storage stability of magnetic disposable immunoplatform was evaluated. The developed immunosensor was compared with the ELISA method.
性别决定区Y框蛋白2（SOX2）是一种转录因子蛋白。SOX2的表达与结直肠癌的淋巴结转移和远处转移有关。使用首个一次性磁性免疫平台测定SOX2。设计的夹心型免疫复合物由捕获抗体、SOX2蛋白和生物素化二抗（dAb/HRP）形成。夹心型免疫复合物与羧酸功能化磁珠（HOOC-MBs）相连。将这种磁性生物共轭物滴在丝网印刷碳电极（SPCE）表面。在对银伪参比电极存在对苯二酚（HQ）和HO的情况下，于-0.20 V进行安培测量。评估了影响免疫分析响应的优化参数。对用于安培检测SOX2标准品的一次性磁性免疫平台进行了分析评估。所开发的免疫传感器显示出高灵敏度（检测限为1.37 ng/mL）和较短的分析时间（15分钟）。对血清样本中发现的潜在干扰化合物进行了测试。评估了一次性磁性免疫平台的储存稳定性。将所开发的免疫传感器与酶联免疫吸附测定（ELISA）方法进行了比较。
