Zhang Gangyu, Moya Adrien, Scherberich Arnaud, Martin Ivan
Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland.
Department of Biomedical Engineering, University of Basel, Allschwil, Switzerland.
Front Bioeng Biotechnol. 2025 Mar 4;13:1550713. doi: 10.3389/fbioe.2025.1550713. eCollection 2025.
Several cartilage and bone organoids have been developed and using adult mesenchymal stromal/stem cells (MSCs) or pluripotent stem cells (PSCs) to mimic different phases of endochondral ossification (ECO), as one of the main processes driving skeletal development and growth. While cellular and molecular features of growth plate-like structures have been observed through the generation and implantation of hypertrophic cartilage tissues, no functional analogue or model of the growth plate has yet been engineered. Herein, after a brief introduction about the growth plate architecture and function, we summarize the recent progress in dissecting the biology of the growth plate and indicate the knowledge gaps to better understand the mechanisms of its development and maintenance. We then discuss how this knowledge could be integrated with state-of-art bioengineering approaches to generate a functional growth plate model.
已经开发了几种软骨和骨类器官，并使用成人间充质基质/干细胞（MSC）或多能干细胞（PSC）来模拟软骨内骨化（ECO）的不同阶段，ECO是驱动骨骼发育和生长的主要过程之一。虽然通过生成和植入肥大软骨组织观察到了生长板样结构的细胞和分子特征，但尚未构建出具有功能的生长板类似物或模型。在此，在简要介绍生长板的结构和功能后，我们总结了剖析生长板生物学的最新进展，并指出了知识空白，以便更好地理解其发育和维持机制。然后，我们讨论如何将这些知识与最新的生物工程方法相结合，以生成具有功能的生长板模型。
