Gao Manman, Liu Xizhe, Guo Peng, Wang Jianmin, Li Junhong, Wang Wentao, Stoddart Martin J, Grad Sibylle, Li Zhen, Wu Huachuan, Li Baoliang, He Zhongyuan, Zhou Guangqian, Liu Shaoyu, Zhu Weimin, Chen Dafu, Zou Xuenong, Zhou Zhiyu
Department of Orthopaedic Surgery, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, China.
Department of Sport Medicine, Institute of Translational Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen 518035, China.
iScience. 2022 Dec 13;26(1):105808. doi: 10.1016/j.isci.2022.105808. eCollection 2023 Jan 20.
The early postnatal limb developmental progression bridges embryonic and mature stages and mirrors the pathological remodeling of articular cartilage. However, compared with multitudinous research on embryonic limb development, the early postnatal stage seems relatively unnoticed. Here, a systematic work to portray the postnatal limb developmental landscape was carried out by characterization of 19,952 single cells from murine hindlimbs at 4 postnatal stages using single-cell RNA sequencing technique. By delineation of cell heterogeneity, the candidate progenitor sub-clusters marked by and were discovered in articular cartilage and enthesis, and three cellular developmental branches marked by , , and were reflected in growth plate. The representative transcriptomes and developmental patterns were intensively explored, and the key regulation mechanisms as well as evolvement in osteoarthritis were discussed. Above all, these results expand horizons of postnatal limb developmental biology and reach the interconnections between limb development, remodeling, and regeneration.
出生后早期肢体发育进程连接胚胎期和成熟期，并反映关节软骨的病理重塑。然而，与众多关于胚胎肢体发育的研究相比，出生后早期阶段似乎相对未被关注。在此，利用单细胞RNA测序技术对出生后4个阶段小鼠后肢的19952个单细胞进行表征，开展了一项描绘出生后肢体发育全景的系统性工作。通过描绘细胞异质性，在关节软骨和附着点中发现了以 和 标记的候选祖细胞亚群，在生长板中反映出以 、 和 标记的三个细胞发育分支。深入探究了代表性转录组和发育模式，并讨论了骨关节炎中的关键调控机制以及演变。最重要的是，这些结果拓展了出生后肢体发育生物学的视野，揭示了肢体发育、重塑和再生之间的联系。
