Hu Qianhua, Li Xiang, Wang Ping, Xie Ying
Key Laboratory of Molecular Epidemiology of Hunan Province, School of Public Health, Health Science Center, Hunan Normal University, Changsha, China.
Med Oncol. 2025 Mar 19;42(4):119. doi: 10.1007/s12032-025-02658-9.
This study explores the roles of ubiquitin-like modification genes in pan-cancer, focusing on their regulatory mechanisms, prognostic implications, and drug sensitivity. Data on five key neddylation pathway genes (RBX1, NEDD8, NAE1, UBA3, UBE2M) were collected from TCGA and GTEx databases, covering mRNA expression, DNA methylation, SNVs, and CNVs. Gene expression differences between normal and cancer tissues, along with associations with genetic alterations, methylation, and cancer-related pathways, were analyzed. Drug sensitivity correlations were assessed using GDSC and CTRP databases. Neddylation pathway genes exhibit hypomethylation and overexpression across various cancers, correlating with poor prognosis. SNVs are predominantly missense mutations, while CNVs are mostly heterozygous deletions and amplifications. These genes regulate several key cancer-related pathways, such as DNA damage repair, cell cycle modulation, and inhibition of RTK/RAS/MAPK pathways. Ubiquitin-like modification genes are associated with poor prognosis due to their low methylation and high expression in cancers. Their genetic alterations impact cancer pathways, underscoring their potential as therapeutic targets and prognostic biomarkers.
本研究探讨类泛素修饰基因在泛癌中的作用，重点关注其调控机制、预后意义和药物敏感性。从TCGA和GTEx数据库收集了五个关键的NEDD化途径基因（RBX1、NEDD8、NAE1、UBA3、UBE2M）的数据，涵盖mRNA表达、DNA甲基化、单核苷酸变异（SNV）和拷贝数变异（CNV）。分析了正常组织和癌组织之间的基因表达差异，以及与基因改变、甲基化和癌症相关途径的关联。使用GDSC和CTRP数据库评估药物敏感性相关性。NEDD化途径基因在各种癌症中表现出低甲基化和过表达，与不良预后相关。SNV主要为错义突变，而CNV大多为杂合缺失和扩增。这些基因调控多个关键的癌症相关途径，如DNA损伤修复、细胞周期调节以及RTK/RAS/MAPK途径的抑制。类泛素修饰基因因其在癌症中的低甲基化和高表达而与不良预后相关。它们的基因改变影响癌症途径，凸显了它们作为治疗靶点和预后生物标志物的潜力。
