Kusonpan Preeya, Kunpatee Kanjana, Chailapakul Orawon, Kalcher Kurt, Ortner Astrid, Chaiyo Sudkate, Samphao Anchalee
Department of Chemistry, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani, 34190, Thailand.
Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Patumwan, Bangkok, 10330, Thailand.
Talanta. 2025 Sep 1;292:127919. doi: 10.1016/j.talanta.2025.127919. Epub 2025 Mar 11.
A user-friendly, rotational paper-based device integrating electrochemical sensors is presented. This innovative device accurately determines nitrite and nitrate ions. Its circular design facilitates the simultaneous detection of both ions. The rotational analytical platform consists of a paper disc that accommodates samples introduced via plastic pipettes. The front side of the disc contains the electrochemical sensors, while the back features a pattern for sample loading. These sensors are specifically designed to detect nitrite and nitrate ions in the sample. Nitrite is determined directly at a working potential of +0.6 V, whereas nitrate is measured after its reduction to nitrite using zinc dust. Moreover, the sensing electrodes are screen-printed carbon electrodes modified with N-doped multiwalled carbon nanotubes (N-MWCNTs) and copper(II) phthalocyanine (CuPc) to enhance sensitivity for nitrite oxidation. This simple device provides a linear range of 50-1000 μM for the simultaneous determination of nitrite and nitrate ions, with detection limits of 10.0 and 20.0 μM, respectively. Furthermore, a manually rotated acrylic plate is employed during sample preparation to facilitate the reduction of nitrate to nitrite. The analytical protocol has been successfully applied to meat samples, and this integrated, portable device shows great potential for rapid, on-site determination of nitrite and nitrate ions in food samples.
本文介绍了一种集成电化学传感器的用户友好型旋转纸质装置。这种创新装置能够准确测定亚硝酸根离子和硝酸根离子。其圆形设计便于同时检测这两种离子。旋转分析平台由一个纸盘组成，纸盘用于容纳通过塑料移液管引入的样品。纸盘正面包含电化学传感器，背面有样品加载图案。这些传感器专门设计用于检测样品中的亚硝酸根离子和硝酸根离子。亚硝酸根离子在 +0.6 V 的工作电位下直接测定，而硝酸根离子则在使用锌粉将其还原为亚硝酸根离子后进行测量。此外，传感电极是用氮掺杂多壁碳纳米管（N-MWCNTs）和铜（II）酞菁（CuPc）修饰的丝网印刷碳电极，以提高对亚硝酸根离子氧化的灵敏度。这种简单的装置在同时测定亚硝酸根离子和硝酸根离子时的线性范围为 50 - 1000 μM，检测限分别为 10.0 和 20.0 μM。此外，在样品制备过程中使用手动旋转的丙烯酸板，以促进硝酸根离子还原为亚硝酸根离子。该分析方法已成功应用于肉类样品，这种集成的便携式装置在快速现场测定食品样品中的亚硝酸根离子和硝酸根离子方面显示出巨大潜力。
