骨桥蛋白（BGN）在透明细胞肾细胞癌（ccRCC）进展和转移中的机制研究

Study on the mechanism of BGN in progression and metastasis of ccRCC.

作者信息

Xia Hanqing, He Tianzhen, Li Xueyu, Zhao Kai, Zhang Zongliang, Zhu Guanqun, Yang Han, Yan Xuechuan, Wang Qinglei, Li Zhaofeng, Jiang Zaiqing, Wang Ke, Yin Xinbao

机构信息

Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, Shangdong, China.

Institute of Special Environmental Medicine, Nantong University, Nantong, China.

出版信息

BMC Med Genomics. 2025 Mar 19;18(1):55. doi: 10.1186/s12920-025-02124-5.

DOI:10.1186/s12920-025-02124-5

PMID:40108593

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11924620/

Abstract

PURPOSE

To investigate the role of Biglycan(BGN) in the progression and metastasis of clear cell renal cell carcinoma(ccRCC).

METHODS

Based on multiple public databases, we investigated the expression level of BGN in ccRCC, its clinical significance, and its association with immune cells. Real-time fluorescence quantitative polymerase chain reaction(PCR) was employed to validate BGN expression in tumor and adjacent normal tissues from ten patients. We utilized RNA sequencing results for further analysis, including differential gene analysis, GO-KEGG analysis, and GSEA analysis, to identify the signaling pathways through which BGN exerts its effects. BGN knockdown cells(786-0 and Caki-1) were generated through lentiviral transfection to examine the impact of BGN on ccRCC. Cell proliferation, migration, and invasion were assessed using CCK8, colony formation, wound healing, Transwell migration, and invasion assays, respectively.

RESULTS

Our findings from database analysis and PCR revealed a significant upregulation of BGN expression in kidney cancer tissues compared to normal tissues. Further analysis demonstrated a correlation between high BGN expression and ccRCC progression and immune infiltration. In vitro experiments confirmed that BGN silencing effectively inhibited cell proliferation, migration, and invasion of ccRCC. Mechanistically, these effects may be mediated through the MAPK signaling pathway.

CONCLUSION

BGN potentially plays a pivotal role in the progression and metastasis of ccRCC, possibly acting through the MAPK signaling pathway. Therefore, BGN holds promise as a potential therapeutic target for ccRCC.

摘要

目的

探讨双糖链蛋白聚糖（BGN）在透明细胞肾细胞癌（ccRCC）进展和转移中的作用。

方法

基于多个公共数据库，我们研究了BGN在ccRCC中的表达水平、其临床意义及其与免疫细胞的关联。采用实时荧光定量聚合酶链反应（PCR）验证10例患者肿瘤组织和癌旁正常组织中BGN的表达。我们利用RNA测序结果进行进一步分析，包括差异基因分析、GO-KEGG分析和GSEA分析，以确定BGN发挥作用的信号通路。通过慢病毒转染构建BGN敲低细胞（786-0和Caki-1），以研究BGN对ccRCC的影响。分别使用CCK8、集落形成、伤口愈合、Transwell迁移和侵袭实验评估细胞增殖、迁移和侵袭能力。