Response surface methodology to optimize the var. liquid fermentation process for the production of fibrinolytic enzyme.

UNLABELLED

Previously, during the (SSP, Dandouchi in China) concoction process, three types of fibrinolytic enzyme-producing bacteria were screened and identified: , and . The fibrin plate approach was used to measure the fibrinolytic enzyme activity of pure fermentation broth, it was found that fibrinolytic enzyme produced by has the highest enzyme activity, followed by and is the lowest. In this study, in order to improve enzyme activity and yield, the response surface method was used to optimize the fermentation conditions, including 6 factors such as nitrogen source, carbon source, initial pH, inoculum amount, loading volume and fermentation temperature. Then, the results showed that: the optional fermentation temperature was determined as 28 ℃, 6% inoculum amount, 30.42 h fermentation time and 20 mL of loading volume in 100 mL erlenmeyer flask. Ultimately, under optimal fermentation conditions, enzyme activity produced by var. SSP was 360.82 IU/mL, which was an increase of 109.68% (1.10 folds) compared to before optimization.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s13197-024-06051-8.