Wang Xingxing, Chen Mengzhen, Lu Ye, Yu Peihao, Zhang Chen, Huang Chao, Yang Zhibiao, Chen Yan, Zhou Jian-Cang
Department of Critical Care Medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, China.
Front Med (Lausanne). 2025 Mar 5;12:1522186. doi: 10.3389/fmed.2025.1522186. eCollection 2025.
This study aimed to investigate the impact of cold atmospheric-pressure plasma (CAP) on multidrug-resistant (MDR) bacteria on various surfaces under nosocomial circumstances and the underlying mechanism.
Four common MDR bacteria (carbapenem-resistant , carbapenem-resistant , methicillin-resistant , and carbapenem-resistant ) were inoculated on nosocomial surfaces, which were subsequently exposed to CAP. Then the bacteria from surfaces were recovered and diluted. The killing curve was analyzed to evaluate the sterilization effects of CAP. Electron microscopy was used to evaluate the changes in cell morphology.
In the CAP-producing device, most of the MDR bacteria were nearly inactivated after 2 h of CAP treatment. Under the simulated ward, CAP exerted an inactivating effect on MDR bacteria. Scanning electron microscopy revealed that the surface of MDR bacteria became blurred, the bodies ruptured and adhered to each other after CAP treatment. The cell walls were thinner as revealed by transmission electron microscopy.
CAP could inactivate the most common MDR bacteria on nosocomial surfaces in simulation ward settings by destroying the structure of pathogens. Our data provided insights into the sterilization of MDR bacteria using CAP and suggested a novel in-hospital disinfection alternative.
本研究旨在调查冷大气压等离子体（CAP）在医院环境下对各种表面上的多重耐药（MDR）细菌的影响及其潜在机制。
将四种常见的多重耐药细菌（耐碳青霉烯类 、耐碳青霉烯类 、耐甲氧西林 、耐碳青霉烯类 ）接种在医院表面，随后将其暴露于CAP。然后从表面回收并稀释细菌。分析杀灭曲线以评估CAP的杀菌效果。使用电子显微镜评估细胞形态的变化。
在产生CAP的装置中，大多数多重耐药细菌在CAP处理2小时后几乎失活。在模拟病房环境下，CAP对多重耐药细菌有灭活作用。扫描电子显微镜显示，多重耐药细菌表面变得模糊，菌体破裂并在CAP处理后相互粘附。透射电子显微镜显示细胞壁变薄。
CAP可通过破坏病原体结构使模拟病房环境下医院表面最常见的多重耐药细菌失活。我们的数据为使用CAP对多重耐药细菌进行消毒提供了见解，并提出了一种新型的医院内消毒替代方法。
