Shi Yan, Yang Yue, Song Yu, Zhu Yujie, Zhao Guoping, Tang Biao
Collaborative Innovation Center for Genetics and Development, State Key Laboratory of Genetic Engineering, Shanghai Engineering Research Center of Industrial Microorganisms, Department of Microbiology, School of Life Sciences, Fudan University, Shanghai, China.
Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, Hangzhou, University of Chinese Academy of Sciences, China.
J Glob Antimicrob Resist. 2025 May;42:222-228. doi: 10.1016/j.jgar.2025.03.008. Epub 2025 Mar 18.
Carbapenems are considered to be the last resort for serious infections caused by multidrug-resistant Gram-negative bacteria, and the emergence of carbapenem-resistant Enterobacteriaceae has posed a serious threat to human health. However, carbapenem resistance is rarely reported in Escherichia fergusonii. In this study, a New Delhi metallo-β-lactamase (NDM)-5-producing E. fergusonii strain, EFSXRJ10, was isolated from a chicken in China.
Minimal inhibitory concentrations were determined using broth microdilution-based antimicrobial susceptibility testing. The complete genome sequence of the NDM-positive isolate was obtained using the Illumina NovaSeq and Oxford Nanopore GridION sequencing platforms, followed by hybrid assembly with Unicycler. In the plasmid conjugation assay, a sodium azide-resistant Escherichia coli strain, J53, was employed as the recipient.
Strain EFSXRJ10 was resistant to ampicillin, amoxycillin-clavulanic acid, gentamicin, spectinomycin, tetracycline, florfenicol, sulfafurazole, cefotaxime, ceftazidime, apramycin and meropenem. The bla gene was located on the IncHI2 plasmid, which can be transferred by conjugation at a frequency of (4.78 ± 0.67) × 10. The bla-carrying plasmid, which harbours 14 antibiotic resistance genes belonging to the IncHI2/ST3 type and exhibiting high similarity to other bla-carrying IncHI2 plasmids, was deposited in GenBank. The genetic structure containing bla was organised as 'IS3000-ΔISAba125-IS5-ΔISAba125-bla-ble-trpF-dsbD-IS26-∆umuD-∆ISKox3-∆IS3000'.
This is the first report characterising the bla-bearing IncHI2 plasmid in E. fergusonii. Surveillance and control measures should be implemented to halt transmission of bla in food animals.
碳青霉烯类药物被认为是治疗多重耐药革兰氏阴性菌引起的严重感染的最后手段，而耐碳青霉烯类肠杆菌科细菌的出现对人类健康构成了严重威胁。然而，弗格森埃希菌对碳青霉烯类药物耐药的情况鲜有报道。在本研究中，从中国一只鸡身上分离出一株产新德里金属β-内酰胺酶（NDM）-5的弗格森埃希菌菌株EFSXRJ10。
采用基于肉汤微量稀释法的药敏试验测定最低抑菌浓度。使用Illumina NovaSeq和牛津纳米孔GridION测序平台获得NDM阳性分离株的完整基因组序列，随后用Unicycler进行混合组装。在质粒接合试验中，使用耐叠氮化钠的大肠杆菌菌株J53作为受体。
菌株EFSXRJ10对氨苄西林、阿莫西林-克拉维酸、庆大霉素、壮观霉素、四环素、氟苯尼考、磺胺异恶唑、头孢噻肟、头孢他啶、阿普拉霉素和美罗培南耐药。bla基因位于IncHI2质粒上，该质粒可通过接合以（4.78±0.67）×10的频率转移。携带bla的质粒含有14个属于IncHI2/ST3型的抗生素抗性基因，与其他携带bla的IncHI2质粒具有高度相似性，已保藏于GenBank。包含bla的基因结构组织为“IS3000-ΔISAba125-IS5-ΔISAba125-bla-ble-trpF-dsbD-IS26-∆umuD-∆ISKox3-∆IS3000”。
这是首次对弗格森埃希菌中携带bla的IncHI2质粒进行特征描述的报道。应实施监测和控制措施以阻止bla在食用动物中的传播。
