Osei Isaac, Wutor Baleng Mahama, Kuyateh Alieu, Barjo Ousman, Sarwar Golam, Omotosho Mayowa, Adefila Williams Oluwatosin, Olawale Yusuf Abdulsalam, Lamin Keita Modou, Hossain Ilias, Lobga Babila G, Wally Muhammed, Cham Morr, Molfa Minteh, Salaudeen Rasheed, Mackenzie Grant A
Medical Research Council Unit The Gambia at London School of Hygiene & Tropical Medicine, Banjul, The Gambia.
Department of Disease Control, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK.
IJID Reg. 2025 Feb 18;14:100606. doi: 10.1016/j.ijregi.2025.100606. eCollection 2025 Mar.
There is a lack of data on the time to blood culture positivity (TTP) in pediatric populations in low-income countries. We aimed to assess the host and pathogen factors associated with TTP in children aged under 5 years in rural Gambia.
Between September 2019 and December 2023, we collected blood cultures from children under 5 years with suspected bloodstream infections. We determined the TTP from the time of culture incubation to when bacterial growth was first detected.
Overall, 547 invasive bacteria pathogens were evaluated. The median TTP was 19.2 hours and 70%, 76%, 89%, and 96% of cases had TTP at 24, 36, 48, and 72 hours, respectively. had the shortest median TTP (17.4 hours), whereas species had the longest (45 hours). TTP was dependent on the pathogen and independent of age, sex, temperature, clinical outcome, nutritional status, and length of hospital stay. Gram-positive bacteria had shorter TTP than gram-negative bacteria (18.6 vs 19.6 hours, < 0.01).
In rural Gambia, most blood cultures from pediatric patients would turn positive within 48 hours of incubation. A maximum of 48 hours of observation after the commencement of antibiotic therapy in hospitalized children may be sufficient for clinicians to receive feedback on blood culture results.
低收入国家儿童血培养阳性时间（TTP）的数据匮乏。我们旨在评估冈比亚农村地区5岁以下儿童中与TTP相关的宿主和病原体因素。
2019年9月至2023年12月期间，我们收集了疑似血流感染的5岁以下儿童的血培养样本。我们确定了从培养孵育开始到首次检测到细菌生长的TTP。
总体而言，共评估了547种侵袭性细菌病原体。TTP的中位数为19.2小时，分别有70%、76%、89%和96%的病例在24、36、48和72小时出现TTP。 的TTP中位数最短（17.4小时），而 菌属的TTP最长（45小时）。TTP取决于病原体，与年龄、性别、体温、临床结局、营养状况和住院时间无关。革兰氏阳性菌的TTP比革兰氏阴性菌短（18.6小时对19.6小时， <0.01）。
在冈比亚农村地区，大多数儿科患者的血培养在孵育48小时内会转为阳性。住院儿童开始抗生素治疗后最多观察48小时，可能足以让临床医生获得血培养结果的反馈。
