LukF-PV在孢子上的表面展示用于口服给药。
Surface display of LukF-PV on spore for oral administration.
作者信息
Nguyen Nhi Ny, Duong Lan, Doan Minh B, Nguyen Hoang Duc
机构信息
Center for Bioscience and Biotechnology, University of Science, Ho Chi Minh City, Vietnam.
Vietnam National University, Ho Chi Minh City, Vietnam.
出版信息
Future Microbiol. 2025 Apr;20(6):469-477. doi: 10.1080/17460913.2025.2480933. Epub 2025 Mar 21.
AIM
spores are promising candidates for oral vaccine, serving as carriers for foreign proteins from pathogens. Here we utilized CotB, a spore coat protein, to anchor the LukF-PV from onto the surface of spores. Our goal was to generate a new strain and assess the capacity of the recombinant spore strain to induce antibody production in mice.
METHODS
The fusion sequence of was cloned into and transformed into HT800F. Colony PCR confirmed the generation of the recombinant strain. SporeELISA verified the display of LukF-PV. Mice were orally gavaged with the spore, and the production of IgA antibodies in feces and IgG in blood was evaluated.
RESULTS
We generated a new strain, BsHT2332, that integrated the P into its genome at locus. BsHT2332 successfully displayed LukF-PV on the spore surface. The recombinant spores induced significant IgA and IgG immune responses in mice.
CONCLUSION
This study demonstrated that spores expressing the antigen LukF-PV can induce an immune response. These findings underscore the potential of the spore platform as a promising approach for vaccine development against .
目的
芽孢有望成为口服疫苗的候选物，可作为病原体外源蛋白的载体。在此，我们利用芽孢衣壳蛋白CotB将来自金黄色葡萄球菌的LukF-PV锚定在芽孢表面。我们的目标是构建一种新的芽孢杆菌菌株，并评估重组芽孢菌株在小鼠体内诱导抗体产生的能力。
方法
将融合序列克隆到载体中并转化到枯草芽孢杆菌HT800F中。菌落PCR证实了重组菌株的产生。芽孢酶联免疫吸附测定（SporeELISA）验证了LukF-PV的展示。用芽孢对小鼠进行口服灌胃，并评估粪便中IgA抗体和血液中IgG的产生情况。
结果
我们构建了一种新的芽孢杆菌菌株BsHT2332，它在特定基因座将P整合到其基因组中。BsHT2332成功地在芽孢表面展示了LukF-PV。重组芽孢在小鼠体内诱导了显著的IgA和IgG免疫反应。
结论
本研究表明，表达金黄色葡萄球菌抗原LukF-PV的芽孢杆菌芽孢可诱导免疫反应。这些发现强调了芽孢杆菌芽孢平台作为一种有前景的抗金黄色葡萄球菌疫苗开发方法的潜力。
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