Chen Shangwen, Tao Caiyan, Huang Yawen, Zhao Ze, Miao Song, Peng Dengfeng, Chen Yashu, Zhou Bin, Deng Ziyu, Deng Qianchun
Key Laboratory of Oilseeds Processing, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, China; School of Life and Health Sciences, Hubei University of Technology, Wuhan, Hubei 430068, China.
Hubei Key Laboratory of Biomass Resource Chemistry and Environmental Biotechnology, Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Engineering Center of Natural Polymers-Based Medical Materials, School of Resource and Environmental Science, Wuhan University, Wuhan, Hubei 430072, China.
Food Chem. 2025 Jul 15;480:143561. doi: 10.1016/j.foodchem.2025.143561. Epub 2025 Mar 11.
Protein glutaminase (PGase) can improve plant protein solubility, but its activity tends to decline under the influence of external factors. Here, we developed a novel PGase-stabilizing agent (sunflower pollen microgel, SPMG) and investigated the mechanism for its stabilizing effect on PGase. Alkali treatment could regulate the physicochemical microenvironment of SPMG, and its ability to stabilize PGase declined with prolonged treatment time. SPMG increased PGase activity by a maximum of 49.24 %, while enhanced its storage stability by 30.61 %, 21.64 %, and 26.00 % at 4 °C, 25 °C, and 37 °C, respectively. SPMG improved PGase properties through hydrophobic interaction, resulting in the burying of inner hydrophobic groups and enhancement of intermolecular hydrogen bonding, which promoted the α-helix content from 23.28 % to 26.19 %. Additionally, these interactions facilitated the sulfhydryl-disulfide bond exchange reaction between PGase molecules, significantly increasing the disulfide bond content by nearly 80 %. This compact structure ultimately enhanced the activity and stability of PGase.
蛋白质谷氨酰胺酶（PGase）可以提高植物蛋白的溶解性，但其活性在外部因素的影响下往往会下降。在此，我们开发了一种新型的PGase稳定剂（向日葵花粉微凝胶，SPMG），并研究了其对PGase稳定作用的机制。碱处理可以调节SPMG的物理化学微环境，且随着处理时间的延长，其稳定PGase的能力会下降。SPMG使PGase活性最高提高了49.24%，同时在4℃、25℃和37℃下分别将其储存稳定性提高了30.61%、21.64%和26.00%。SPMG通过疏水相互作用改善了PGase的性质，导致内部疏水基团被掩埋，分子间氢键增强，使α-螺旋含量从23.28%提高到26.19%。此外，这些相互作用促进了PGase分子间的巯基-二硫键交换反应，使二硫键含量显著增加了近80%。这种紧密结构最终增强了PGase的活性和稳定性。
