Deniz Ahmet Tolga, Büyükkaplan Ulviye Şebnem, Gümüş Burçin Aşkım, Daltaban Özlem, Türker Nurullah
Researcher, Ministry of Health, Antalya, Turkey.
Professor, Department of Prosthodontics, Faculty of Dentistry, Akdeniz University, Antalya, Turkey.
J Prosthet Dent. 2025 Jul;134(1):178.e1-178.e7. doi: 10.1016/j.prosdent.2025.02.059. Epub 2025 Mar 20.
While dual-polymerizing self-adhesive resin cements have been widely used because of their bonding capabilities and ease of use, there is a lack of comprehensive data on their biocompatibility, particularly concerning the cytotoxic effects of different polymerization methods on cell viability.
The purpose of this in vitro study was to investigate the potential cytotoxic effects of 3 different dual-polymerizing self-adhesive resin cements polymerized by light polymerization or autopolymerization on L929 cells in vitro using by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) test.
Three different dual-polymerizing self-adhesive resin cements (Calibra Universal; Dentsply Sirona Inc, SpeedCEM Plus; Ivoclar AG, and TheraCem Ca; Bisco Inc) were light or autopolymerized in polytetrafluoroethylene (PTFE) molds containing Ø5-mm and 2-mm-thick cells in accordance to the manufacturer's instructions. The specimens were incubated in Dulbecco Modified Eagle Medium (DMEM-High; Capricorn Scientific GmbH) for 48 hours and the extracts were obtained. The 100% concentration of the extract was diluted and extracts at 66.7%, 44.4%, 29.6%, and 19.8% concentrations were obtained. Specimen extracts at 5 different concentrations were incubated with L929 (NCTC clone 929: CCL 1; American Type Culture Collection) mouse fibroblast cells in 96-well tissue culture plates at 37 °C and 5% CO for 24, 48, and 72 hours. At the end of the incubation period, the effects of the materials on cell viability were evaluated with the MTT test. The data were analyzed using a statistical software program (IBM SPSS Statistics, v25.0; IBM Corp) (α=.05), employing ANOVA and the Tukey's HSD test.
All tested cement specimens significantly reduced cell viability (P<.05). Cell viability decreased with increasing concentration and incubation time in all specimens tested. The light-polymerized SpeedCem Plus showed the least cytotoxicity regardless of concentration and incubation time, followed by TheraCem Ca. However, the autopolymerized Calibra Universal significantly reduced cell viability. Cell viability rate of all light polymerized cements was considerably higher than autopolymerized cements (P<.05).
All the tested self-adhesive resin cements caused a significant reduction in viability of L929 cells. The composition of the self-adhesive resin cement and the activation type of polymerization affected cytotoxicity.
虽然双固化自粘树脂水门汀因其粘结能力和易用性而被广泛使用，但关于其生物相容性的综合数据却很缺乏，尤其是不同聚合方法对细胞活力的细胞毒性作用方面。
本体外研究的目的是通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）试验，研究3种不同的双固化自粘树脂水门汀通过光固化或自固化聚合后对体外L929细胞的潜在细胞毒性作用。
3种不同的双固化自粘树脂水门汀（Calibra Universal；登士柏西诺德公司，SpeedCEM Plus；义获嘉伟瓦登特公司，以及TheraCem Ca；必思科公司）按照制造商的说明在含有直径5毫米和2毫米厚细胞的聚四氟乙烯（PTFE）模具中进行光固化或自固化。将标本在杜尔贝科改良伊格尔培养基（DMEM-High；摩羯座科学有限公司）中孵育48小时，然后获得提取物。将提取物的100%浓度进行稀释，得到浓度为66.7%、44.4%、29.6%和19.8%的提取物。将5种不同浓度的标本提取物与L929（NCTC克隆929：CCL 1；美国典型培养物保藏中心）小鼠成纤维细胞在96孔组织培养板中于37°C和5%二氧化碳条件下孵育24、48和72小时。在孵育期结束时，用MTT试验评估材料对细胞活力的影响。使用统计软件程序（IBM SPSS Statistics，v25.0；IBM公司）（α = 0.05）进行数据分析，采用方差分析和Tukey's HSD检验。
所有测试的水门汀标本均显著降低细胞活力（P < 0.05）。在所有测试标本中，细胞活力随浓度和孵育时间的增加而降低。无论浓度和孵育时间如何，光固化的SpeedCem Plus显示出最小的细胞毒性，其次是TheraCem Ca。然而，自固化的Calibra Universal显著降低细胞活力。所有光固化水门汀的细胞活力率均显著高于自固化水门汀（P < 0.05）。
所有测试的自粘树脂水门汀均导致L929细胞活力显著降低。自粘树脂水门汀的组成和聚合的激活类型影响细胞毒性。
