巴西潘塔纳尔湿地南部野生食肉动物和家犬中肝簇虫属及嗜吞噬细胞无形体的分子检测与新宿主记录

Molecular detection of Hepatozoon spp. and Cytauxzoon felis in wild carnivorans and domestic dogs in southern Pantanal wetlands of Brazil with new host records.

作者信息

Alves Mario H, Martins Nathana B, Hora Aline S, Soresini Grazielle, Desbiez Arnaud L J, Mendoza-Roldan Jairo Alfonso, Otranto Domenico, Paiva Fernando

机构信息

Instituto de Conservação de Animais Silvestres-ICAS, Campo Grande, Mato Grosso Do Sul, Brazil; Laboratory of Animal Parasitology, Institute of Biosciences, Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil; Department of Veterinary Medicine, University of Bari, Bari, Brazil.

Laboratory of Veterinary Etiological Investigation, School of Veterinary Medicine, Federal University of Uberlandia, Uberlandia, Brazil.

出版信息

Vet Parasitol Reg Stud Reports. 2025 Apr;59:101233. doi: 10.1016/j.vprsr.2025.101233. Epub 2025 Mar 10.

DOI:10.1016/j.vprsr.2025.101233

PMID:40121047

Abstract

In this study, we investigated the occurrence of Hepatozoon spp. and Cytauxzoon felis in spleen samples from 26 wild carnivorans and blood samples from 28 semi-domiciled dogs collected in the Pantanal biome of Brazil. Partial 18S rRNA gene sequences of Hepatozoon spp. were detected in 15 (57.7 %) wild carnivorans and 3 (10.7 %) domestic dogs. A sequence obtained from a domestic dog was similar (99.04 %) to that of Hepatozoon canis from a crab-eating fox (Cerdocyon thous) in the same area of Brazil. Moreover, we report the neotropical otter (Lontra longicaudis) and the bush dog (Speothos venaticus) as new hosts for Hepatozoon spp. in free-ranging conditions. Additionally, C. felis was detected in two jaguars (Panthera onca), confirming the occurrence of this protozoan within this felid species, which could play a role in the maintenance of C. felis under natural conditions. Considering the above results, large-scale surveys are recommended to further assess the distribution of vector-borne pathogens and evaluate their virulence and zoonotic potential in wild carnivorans from the Pantanal.

摘要

在本研究中，我们调查了在巴西潘塔纳尔生物群落采集的26只野生食肉动物的脾脏样本以及28只半家养犬的血液样本中肝簇虫属（Hepatozoon spp.）和嗜吞噬细胞无形体（Cytauxzoon felis）的发生情况。在15只（57.7%）野生食肉动物和3只（10.7%）家养犬中检测到肝簇虫属的部分18S rRNA基因序列。从一只家养犬获得的序列与来自巴西同一地区食蟹狐（Cerdocyon thous）的犬肝簇虫（Hepatozoon canis）的序列相似（99.04%）。此外，我们报告了新热带水獭（Lontra longicaudis）和丛林犬（Speothos venaticus）是自由放养条件下肝簇虫属的新宿主。此外，在两只美洲豹（Panthera onca）中检测到嗜吞噬细胞无形体，证实了这种原生动物在该猫科动物物种中的存在，这可能在自然条件下嗜吞噬细胞无形体的维持中发挥作用。考虑到上述结果，建议进行大规模调查，以进一步评估媒介传播病原体的分布，并评估它们在潘塔纳尔野生食肉动物中的毒力和人畜共患病潜力。