Dos Anjos Pacheco Thábata, Lee Daniel Antônio Braga, Maia Maerle Oliveira, Semedo Thiago Borges Fernandes, de Mendonça Ravena Fernanda Braga, Pedroni Fernando, Dutra Valéria, Nakazato Luciano, Rossi Rogério Vieira, André Marcos Rogério, de Campos Pacheco Richard
Programa de Pós-Graduação em Ciências Veterinárias (PPGVET), Faculdade de Medicina Veterinária (FAVET), Universidade Federal de Mato Grosso (UFMT). Av. Fernando Corrêa da Costa, 2367, Boa Esperança, Cuiabá, MT, 78060900, Brazil.
Ciência e Tecnologia de Mato Grosso (IFMT) - Campus Campo novo do Parecis, Instituto Federal de Educação, Av. Vilmar Fernandes, 300, Campo Novo do Parecis, MT, 78652000, Brazil.
Parasitol Res. 2025 Feb 4;124(2):19. doi: 10.1007/s00436-025-08460-w.
The order Piroplasmida (e.g., Babesia, Theileria, Cytauxzoon, and Rangelia) and suborder Adelorina (e.g., Hepatozoon) comprise protozoa of the phylum Apicomplexa, which are related to significant economic, veterinary, and medical concerns. This study aimed to investigate the molecular occurrence and evaluate the phylogenetic placement of the Hepatozoon and Piroplasmida agents in small mammals (rodents and marsupials) in midwestern Brazil. The maximum likelihood phylogenetic inference positioned the 18S ribosomal RNA (18S rRNA) sequences of Hepatozoon obtained from Hylaeamys megacephalus with those previously detected in Didelphis aurita from Brazil; however, the sequences of Hepatozoon retrieved from Nectomys rattus and Neacomys amoenus clustered in a sub-clade sister to a clade of sequences detected in small rodents from Brazil. Phylogenetic inference positioned the piroplasmid 18S rRNA sequences detected in Thrichomys pachyurus in a single sub-clade sister to a clade containing sequences previously detected in the Phyllostomidae bat Phyllostomus discolor from midwestern Brazil. In contrast, the piroplasmid cytochrome b mitochondrial gene (cytB) sequences obtained from T. pachyurus clustered into a single clade. In conclusion, a new host record is provided for Hepatozoon spp. from the rodent N. amoenus. Furthermore, this study expanded the Piroplasmida order diversity by identifying novel genotypes in T. pachyurus rodents, which were genetically related to the piroplasmid clade found in Neotropical bats and named herein the "South American Rodentia group."
梨形虫目（如巴贝斯虫属、泰勒虫属、嗜吞噬细胞无形体属和兰热利属）和无类锥体亚目（如肝簇虫属）包含顶复门原生动物，这些原生动物与重大的经济、兽医和医学问题相关。本研究旨在调查巴西中西部小型哺乳动物（啮齿动物和有袋动物）中肝簇虫属和梨形虫目的分子存在情况，并评估它们的系统发育位置。最大似然系统发育推断将从巨头稻鼠中获得的肝簇虫属的18S核糖体RNA（18S rRNA）序列与先前在巴西的南美袋鼬中检测到的序列进行了定位；然而，从黑腹巢鼠和阿氏姬鼠中检索到的肝簇虫属序列聚集在一个亚分支中，该亚分支是巴西小型啮齿动物中检测到的序列分支的姐妹分支。系统发育推断将在粗毛鼠中检测到的梨形虫18S rRNA序列定位在一个单一的亚分支中，该亚分支是包含先前在巴西中西部叶口蝠科蝙蝠叶口蝠中检测到的序列的分支的姐妹分支。相比之下，从粗毛鼠中获得的梨形虫细胞色素b线粒体基因（cytB）序列聚集在一个单一的分支中。总之，本研究提供了阿氏姬鼠作为肝簇虫属新宿主的记录。此外，通过在粗毛鼠中鉴定出与新热带蝙蝠中发现的梨形虫分支有遗传关系的新基因型，本研究扩展了梨形虫目的多样性，在此将其命名为“南美啮齿动物组”。
