Li Ang, Miao Xin, Han Zhengzhe, Lin Junqing, Huang Jinghuan, Zheng Xianyou
Department of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, No. 600 Yishan Road, Shanghai, 200233, China.
National Center for Orthopaedics, No. 600 Yishan Road, Shanghai, 200233, China.
Mater Today Bio. 2025 Feb 28;31:101624. doi: 10.1016/j.mtbio.2025.101624. eCollection 2025 Apr.
Traumatic spinal cord injury (SCI) is a major clinical challenge, imposing a significant burden on both patients and healthcare systems. The complexity of SCI stems from its multifactorial pathogenesis, incorporating a variety of regulating factors. Despite the exploration of mechanisms of SCI pathophysiology and the development of biomedical therapies, current clinical interventions are still limited to surgical interventions and rehabilitative care. This study introduces an approach to protect mitochondria-a pivotal factor in SCI pathogenesis-through the use of poly(glycerol succinate)-based hydrogel. To regulate the process, the PEGylated poly(glycerol succinate) (PPGS), was designed and synthesized via a novel method, combined with recent findings that emphasize the roles of glycerol-based hydrogel in soft tissue regeneration. Building on these, an innovative, bioenergetically-active hydrogel, acrylated PEGylated poly(glycerol succinate) (APPGS), which improves mitochondrial function after injury, targeting SCI treatment, was developed. The evidence, supported by both in vivo and in vitro assays, affirms the therapeutic efficacy of the APPGS hydrogel in SCI contexts. The APPGS hydrogel represents a significant advancement with substantial potential for clinical application in SCI therapy, offering a new avenue for addressing the complex challenges of SCI management.
创伤性脊髓损伤（SCI）是一项重大临床挑战，给患者和医疗系统都带来了沉重负担。SCI的复杂性源于其多因素发病机制，涉及多种调节因子。尽管对SCI病理生理学机制进行了探索，生物医学疗法也有所发展，但目前的临床干预仍局限于手术干预和康复护理。本研究介绍了一种通过使用基于聚琥珀酸甘油酯的水凝胶来保护线粒体（SCI发病机制中的关键因素）的方法。为了调控这一过程，通过一种新方法设计并合成了聚乙二醇化聚琥珀酸甘油酯（PPGS），结合了近期强调甘油基水凝胶在软组织再生中作用的研究结果。在此基础上，开发了一种创新的、具有生物能量活性的水凝胶——丙烯酸化聚乙二醇化聚琥珀酸甘油酯（APPGS），它可改善损伤后的线粒体功能，用于靶向治疗SCI。体内和体外试验的证据均证实了APPGS水凝胶在SCI情况下的治疗效果。APPGS水凝胶代表了一项重大进展，在SCI治疗的临床应用中具有巨大潜力，为应对SCI管理的复杂挑战提供了一条新途径。
