Yamaguchi Manaho, Fukuyama Yuko, Izumi Shunsuke
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8526, Japan.
Koichi Tanaka Mass Spectrometry Research Laboratory, Shimadzu Corporation, 1 Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan.
Mass Spectrom (Tokyo). 2025;14(1):A0170. doi: 10.5702/massspectrometry.A0170. Epub 2025 Mar 18.
In matrix-assisted laser desorption ionization mass spectrometry, a suitable matrix is often selected for the analyte. Herein, we first developed a novel matrix, alkylated hydroxychalcone (AHC), which has properties similar to alkylated trihydroxyacetophenone (ATHAP) (., 85: 9444-9448, 2013) developed as a matrix for hydrophobic peptides. However, the sample-to-sample reproducibility was low because of the poor crystallinity of AHC. The crystalline morphology of AHC changed when AHC/2,5-dihydroxybenzoic acid (DHB) was used as a binary matrix. As a result, the use of AHC/DHB improved sample-to-sample reproducibility and increased sensitivity for hydrophobic peptides. Mass imaging indicated that these results were due to an increased number of sweet spots wherein the analytes were detected as ion peaks, in a matrix/analyte crystal spot.
在基质辅助激光解吸电离质谱分析中，通常会为分析物选择合适的基质。在此，我们首次开发了一种新型基质——烷基化羟基查耳酮（AHC），其性质与作为疏水性肽基质开发的烷基化三羟基苯乙酮（ATHAP）（《美国化学会志》，85: 9444 - 9448, 2013）相似。然而，由于AHC的结晶性较差，样品间的重现性较低。当AHC/2,5 - 二羟基苯甲酸（DHB）用作二元基质时，AHC的晶体形态发生了变化。结果，使用AHC/DHB提高了样品间的重现性，并增加了对疏水性肽的灵敏度。质谱成像表明，这些结果是由于在基质/分析物晶体斑点中，被检测为离子峰的分析物的“甜点”数量增加所致。
