Herken R
Teratology. 1985 Jun;31(3):345-52. doi: 10.1002/tera.1420310305.
The ultrastructural changes in the neural tube of 10-day-old mouse embryos were investigated between 1.5 hr and 4 hr after application of either 1 mg/kg colchicine (Col) or 500 mg/kg hydroxyurea (HU) or simultaneous application of both substances. During the investigated period, the shape of the nuclei of the neuroepithelial cells had changed from elongated to round after Col application. The chromatin in the nuclei was condensed and arranged in clusters. A breakdown of polysomes into ribosomes and an enlargement of the rough ER was observed in the cytoplasm. At the luminal surface, bleb-like cytoplasmic processes of the neuroepithelial cells containing monoribosomes protruded into the lumen. No cell necroses were visible in the neural tube after Col application. A condensation of chromatin in the nuclei of some neuroepithelial cells was visible 1.5 hr after HU application. Shortly thereafter, cell necroses appeared in the neural tube and 4 hr after HU application the entire spinal cord was strongly damaged. After simultaneous application of Col and HU, the ultrastructural changes in the neuroepithelial cells of the neural tube did not differ from the results obtained after Col application alone. In contrast to the results obtained after HU application alone, no necroses occurred after simultaneous application of Col and HU.
在给10日龄小鼠胚胎施用1mg/kg秋水仙碱（Col）或500mg/kg羟基脲（HU）或同时施用这两种物质后的1.5小时至4小时之间，对神经管的超微结构变化进行了研究。在研究期间，施用Col后，神经上皮细胞核的形状从细长形变为圆形。细胞核中的染色质浓缩并聚集成簇。在细胞质中观察到多核糖体分解为核糖体以及粗面内质网扩大。在管腔表面，含有单核糖体的神经上皮细胞的泡状细胞质突起伸入管腔。施用Col后神经管中未见细胞坏死。施用HU后1.5小时，可见一些神经上皮细胞核中的染色质浓缩。此后不久，神经管中出现细胞坏死，施用HU后4小时，整个脊髓受到严重损伤。同时施用Col和HU后，神经管神经上皮细胞的超微结构变化与单独施用Col后的结果无差异。与单独施用HU后的结果相反，同时施用Col和HU后未发生坏死。
