Jiang Peng, Wu Lei, Li Chang, Hao Yongchao, He Yi, Zhang Peng, Wang Hongwei, Zhang Xu
CIMMYT-JAAS Joint Center for Wheat Diseases/The Research Center of Wheat Scab/Key Laboratory of Germplasm Innovation in Downstream of Huaihe River (Nanjing) Ministry of Agriculture and Rural Affairs, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Zhongshan Biological Breeding Laboratory, Nanjing, Jiangsu, China.
Plant Dis. 2025 Jul;109(7):1526-1533. doi: 10.1094/PDIS-10-24-2115-RE. Epub 2025 Jul 15.
Fusarium head blight (FHB), a serious disease in wheat, causes significant reductions in grain yield and quality worldwide. Marker-assisted selection can provide an effective tool for improving FHB resistance. Although many quantitative trait loci (QTLs) associated with resistance to FHB have been reported, few loci are currently available in breeding programs. The middle and lower reaches of the Yangtze River in China are areas that historically have experienced FHB epidemics, so that some local varieties may contain some valuable genes providing FHB resistance. In this study, association mapping was performed using 103 locally cultivated varieties, and a previous QTL mapping using a recombinant inbred line population from two locally popular parents, specifically Ningmai 9 and Yangmai 158, was integrated to identify nine candidate intervals. The corresponding kompetitive allele-specific PCR markers associated with FHB resistance were successfully developed and validated in 611 breeding lines. Four loci, namely , , , and , proved to be significantly related to FHB resistance in addition to , and they could provide good resistance to FHB in the absence of ; the combination of multiple loci could produce a more stable resistance. These five markers were then applied in 211 breeding lines, and many resistance lines were obtained with different combinations of resistance loci. Additionally, proved to be a highly selected locus, and eight differential genes in its interval were identified by genome and transcriptome analysis. This study provides additional gene resources and materials that could be used in FHB resistance breeding in wheat.
小麦赤霉病（FHB）是小麦的一种严重病害，在全球范围内导致谷物产量和品质大幅下降。标记辅助选择可为提高小麦对赤霉病的抗性提供有效工具。尽管已报道了许多与抗赤霉病相关的数量性状位点（QTL），但目前育种计划中可用的位点很少。中国长江中下游地区是历史上赤霉病流行的地区，因此一些当地品种可能含有一些提供抗赤霉病的宝贵基因。在本研究中，利用103个当地栽培品种进行了关联作图，并整合了之前使用两个当地流行亲本（宁麦9号和扬麦158）的重组自交系群体进行的QTL作图，以确定9个候选区间。成功开发了与抗赤霉病相关的竞争性等位基因特异性PCR标记，并在611个育种系中进行了验证。除了 、 和 之外，四个位点 、 、 和 被证明与抗赤霉病显著相关，并且在没有 的情况下它们能提供良好的抗赤霉病能力；多个位点的组合可以产生更稳定的抗性。然后将这五个标记应用于211个育种系，通过不同抗性位点组合获得了许多抗性品系。此外， 被证明是一个高度选择的位点，通过基因组和转录组分析在其区间内鉴定出8个差异基因。本研究提供了可用于小麦抗赤霉病育种的额外基因资源和材料。
