Menezes Olga Cedro de, Carvalho Vinícius M De, Queiroz Victor H O de, Rezende Camilla A Do N, Costa Quésia G, Gordiano Layse A, Araújo Maria L G M L de, Ribeiro Cláudio V D M, Netto Arlindo S, Rotta Polyana P, Pina Douglas Dos S, Carvalho Gleidson G P de, Brant Lara M S, Loiola Marcus V G, Freitas Júnior José E De
Department of Animal Science, Federal University of Bahia, Salvador, Brazil.
Department of Animal Science, Faculty of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.
Arch Anim Nutr. 2025 Feb;79(1):27-45. doi: 10.1080/1745039X.2025.2472690. Epub 2025 Mar 26.
The objective of this study was to evaluate the effects of chitosan (CH), with or without the inclusion of unsaturated fatty acid (UFA) sources, on intake and digestibility, ruminal fermentation, microbial protein synthesis, nitrogen balance, blood metabolites, milk production and composition, and ruminal dynamics and flow in lactating Jersey cows. Five rumen-fistulated Jersey cows [average days in milk = 105 ± 45 days; milk production = 18.0 ± 0.47 kg/day; body weight = 350 ± 25 kg] were used in a 5 × 5 Latin square design with 21-day periods. Five experimental diets were evaluated: (1) Control (CON), basal diet including 30 g/kg of palm oil; (2) (SOCH-) inclusion of 30 g/kg soybean oil (SO) without CH; (3) (WSCH-) inclusion of 160 g/kg whole raw soybeans (WS) without CH; (4) (SOCH+) inclusion of 30 g/kg SO and 2 g/kg CH; (5) (WSCH+) inclusion of 160 g/kg WS and 2 g/kg CH. There was a CH and UFA interaction effect on acetate (C2) concentration ( = 0.005), propionate (C3) concentration ( = 0.008), the C2:C3 ratio ( < 0.001), and the milk fat content of trans-9 C18:1 fatty acid (FA) ( = 0.004). The inclusion of SO, with or without CH, proved to be more active in improving ruminal fermentation patterns by increasing propionate concentration. Furthermore, incorporating SO into the diets of lactating Jersey cows increased CLA, particularly -9, -11, in milk fat, offering health benefits. We recommend further research to assess the effects of UFA supplementation in combination with CH.
本研究的目的是评估壳聚糖（CH）添加或不添加不饱和脂肪酸（UFA）来源对泌乳泽西奶牛的采食量和消化率、瘤胃发酵、微生物蛋白合成、氮平衡、血液代谢物、产奶量和组成以及瘤胃动力学和流量的影响。选用5头装有瘤胃瘘管的泽西奶牛[平均泌乳天数 = 105 ± 45天；产奶量 = 18.0 ± 0.47千克/天；体重 = 350 ± 25千克]，采用5×5拉丁方设计，每期21天。评估了五种试验日粮：（1）对照（CON），基础日粮含30克/千克棕榈油；（2）（SOCH-）添加30克/千克大豆油（SO）且不添加CH；（3）（WSCH-）添加160克/千克全生大豆（WS）且不添加CH；（4）（SOCH+）添加30克/千克SO和2克/千克CH；（5）（WSCH+）添加160克/千克WS和2克/千克CH。CH和UFA对乙酸盐（C2）浓度（P = 0.005）、丙酸盐（C3）浓度（P = 0.008）、C2:C3比值（P < 0.001）以及反式-9 C18:1脂肪酸（FA）的乳脂肪含量（P = 0.004）存在交互作用。结果表明，添加SO（无论是否添加CH）通过提高丙酸盐浓度，在改善瘤胃发酵模式方面更具活性。此外，在泌乳泽西奶牛日粮中添加SO可增加乳脂肪中CLA的含量，尤其是-9，-11 CLA，对健康有益。我们建议进一步开展研究，以评估UFA补充剂与CH联合使用的效果。
