Cavaille F
Acta Physiol Hung. 1985;65(4):453-60.
The contractile proteins of the human myometrium were quantified and characterized in order to investigate their possible modifications during pregnancy. The myosin concentration was found to be 1 to 5 mg/g of tissue, while actin concentration ranged from 16 to 60 mg/g, leading to an actin/myosin ratio higher (Mean = 14) than in other smooth muscles. Purified myosin submitted to two-dimensional gel electrophoresis exhibited two isoelectric forms for the 17 KD Light Chain, the more acidic being predominant in the pregnant organ, the more basic in the non gravid one. The mobility of myosin of form filaments was studied using electron microscopy. Only the myosin purified from gravid uteri in its phosphorylated form did aggregate in long bipolar filaments. Actin was characterized in crude muscle extracts using two-dimensional gel electrophoresis. It appeared in three forms differing by their isoelectric points. The more basic form (gamma) predominates in the pregnant organ, as soon as 17 weeks of pregnancy, while in the non-pregnant uterus it is the intermediate (beta) form which is predominant.
为了研究人类子宫肌层收缩蛋白在孕期可能发生的变化，对其进行了定量和特性分析。结果发现，肌球蛋白浓度为每克组织1至5毫克，而肌动蛋白浓度在每克16至60毫克之间，导致肌动蛋白与肌球蛋白的比例（平均值为14）高于其他平滑肌。经二维凝胶电泳分析，纯化后的肌球蛋白在17KD轻链上呈现出两种等电形式，酸性较强的形式在妊娠子宫中占主导，碱性较强的形式在未孕子宫中占主导。利用电子显微镜研究了丝状形式的肌球蛋白的迁移率。只有从妊娠子宫中纯化出的磷酸化形式的肌球蛋白会聚集成长的双极丝。利用二维凝胶电泳对粗制肌肉提取物中的肌动蛋白进行了特性分析。它呈现出三种等电点不同的形式。碱性较强的形式（γ）在妊娠17周后即在妊娠子宫中占主导，而在未孕子宫中占主导的是中间形式（β）。
