Chen Sheng, Zhou Min, Xiao Ximian, Xie Jiayang, Liu Longqiang, Cong Zihao, Zhao Xuebin, Hu Weilong, Wang Jie, Song Gonghua, Liu Runhui
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China.
China Railway Academy Group Co., Ltd., Chengdu, Sichuan 610032, P. R. China.
J Agric Food Chem. 2025 Apr 9;73(14):8191-8203. doi: 10.1021/acs.jafc.4c12430. Epub 2025 Mar 26.
Given the limited types of agricultural bactericides and the rapid emergence of antimicrobial resistance, bacterial plant diseases pose a serious threat to agricultural production, which calls for effective antimicrobial agents with a low propensity for resistance. Host defense peptides (HDPs) have drawn significant attention for their broad-spectrum antimicrobial activity. In this study, we found that HPD-mimicking poly(2-oxazoline) Gly-POX exhibits potent activity against bacterial phytopathogens, with superior antibacterial selectivity and proteolytic stability compared to the natural HDP melittin. Compared to commonly used agricultural bactericides, Gly-POX displays more efficient antibiofilm activity and a lower propensity for resistance than does the antibiotic streptomycin, likely due to its antibacterial mechanism, which involves DNA interaction and generating lethal doses of ROS. studies reveal that Gly-POX is effective in preventing and treating phytopathogens without observable damage to plant tissues, suggesting that poly(2-oxazoline) could be a promising bactericide for agricultural applications.
鉴于农业杀菌剂类型有限以及抗菌药物耐药性的迅速出现，细菌性植物病害对农业生产构成严重威胁，这就需要具有低耐药倾向的有效抗菌剂。宿主防御肽（HDPs）因其广谱抗菌活性而备受关注。在本研究中，我们发现模拟HPD的聚（2-恶唑啉）Gly-POX对细菌性植物病原体具有强大的活性，与天然HDP蜂毒素相比，具有优异的抗菌选择性和蛋白水解稳定性。与常用的农业杀菌剂相比，Gly-POX表现出更高效的抗生物膜活性，并且比抗生素链霉素具有更低的耐药倾向，这可能归因于其抗菌机制，该机制涉及DNA相互作用和产生致死剂量的活性氧。研究表明，Gly-POX在预防和治疗植物病原体方面有效，且对植物组织无明显损害，这表明聚（2-恶唑啉）可能是一种有前途的农业用杀菌剂。
