Wang Qianchao, Zhang Chuanli, Guo Yirui, Liu Junna, Zhang Ping, Jiang Guofei, Qin Peng
College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China.
Int J Mol Sci. 2025 Mar 12;26(6):2542. doi: 10.3390/ijms26062542.
Yunnan hulled wheat (YHW) is a wild ancestor of cultivated wheat and possesses rich genetic variation; however, there is limited research on teff at the molecular level. Therefore, in this study, two lines with large differences in kernel size were used as materials, and their kernels were sampled at 7, 21, 35, and 49 days after flowering; kernel surface area and thousand kernel weight were measured and analyzed; and transcriptome sequencing analysis was also performed, which showed that a total of 88,801 genes were annotated by Kyoto Encyclopedia of Genes and Genomes (KEGG); the functional annotation showed that the key pathways they involved in yield traits were mainly enriched in gycolysis/gluconeogenesis, pentose and glucuronate interconversions, amino sugar and nucleotide sugar metabolism, starch, and sucrose metabolism pathways, of which , , , and differed significantly in different subgroups; and a significant difference was observed between the two pathways in different subgroups using weighted gene co-expression network analysis (WGCNA) associated with yield traits. Ten core genes were mined from the two modules with the highest correlation with the target traits. These results provide a theoretical reference basis for interpreting the expression patterns of yield trait-responsive genes in YHW, for further conservation and utilization of the germplasm resources of this rare wheat, and for the screening of high-yielding superior varieties.
云南裸粒小麦（YHW）是栽培小麦的野生祖先，具有丰富的遗传变异；然而，在分子水平上对云南裸粒小麦的研究有限。因此，本研究以两个粒重差异较大的品系为材料，在开花后7天、21天、35天和49天对其籽粒进行取样；测量并分析了籽粒表面积和千粒重；还进行了转录组测序分析，结果表明，共有88,801个基因被京都基因与基因组百科全书（KEGG）注释；功能注释表明，它们参与产量性状的关键途径主要富集在糖酵解/糖异生、戊糖和葡糖醛酸相互转化、氨基糖和核苷酸糖代谢、淀粉和蔗糖代谢途径中，其中， 、 、 和 在不同亚组中差异显著；使用与产量性状相关的加权基因共表达网络分析（WGCNA）在不同亚组的两条途径之间观察到显著差异。从与目标性状相关性最高的两个模块中挖掘出10个核心基因。这些结果为解析云南裸粒小麦产量性状响应基因的表达模式、进一步保护和利用这种珍稀小麦的种质资源以及筛选高产优良品种提供了理论参考依据。
