Vakhrameev Sergey A, Ovchinnikov Denis V, Ul'yanovskii Nikolay V, Kosyakov Dmitry S
Laboratory of Environmental Analytical Chemistry, Core Facility Center "Arktika", Northern (Arctic) Federal University, Arkhangelsk 163002, Russia.
Molecules. 2025 Mar 10;30(6):1236. doi: 10.3390/molecules30061236.
Nitrogen-containing heterocyclic compounds (NHCs) are common environmental pollutants that need to be monitored due to their high toxicity. Typically, gas or liquid chromatography combined with mass spectrometric detection is used for this task. However, many NHCs are highly polar compounds, which can cause difficulties when using these methods. On the other hand, supercritical fluid chromatography is well-established in the analysis of polar compounds and could provide an alternative to conventional techniques. The presented work proposes an approach to the simultaneous determination of 20 NHCs in soils by supercritical fluid chromatography-tandem mass spectrometry with the limits of quantification in the range 0.08-1.23 mg kg. The separation is carried out in gradient mode on a cyanopropyl stationary phase in 6 min. The approach was validated and tested on real objects-peat and sandy soils contaminated with rocket fuel transformation products.
含氮杂环化合物（NHCs）是常见的环境污染物，因其高毒性而需要进行监测。通常，气相或液相色谱结合质谱检测用于此项任务。然而，许多NHCs是高极性化合物，使用这些方法时会遇到困难。另一方面，超临界流体色谱在极性化合物分析方面已得到广泛应用，可为传统技术提供替代方案。本文提出了一种通过超临界流体色谱-串联质谱同时测定土壤中20种NHCs的方法，定量限在0.08 - 1.23 mg/kg范围内。分离在氰丙基固定相上以梯度模式在6分钟内完成。该方法在受火箭燃料转化产物污染的泥炭和沙质土壤等实际样品上进行了验证和测试。
