Qu Liang, Lu Qiongya, Zhang Liming, Kong Fanzhuo, Zhang Yuyang, Lin Zhiyuan, Ni Xing, Zhang Xue, Zhao Yani, Zou Bin
School of Food and Bioengineering, Wuhu Institute of Technology, Wuhu 241003, China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Molecules. 2025 Mar 10;30(6):1241. doi: 10.3390/molecules30061241.
Vitamin E succinate is a more mature vitamin E derivative, and its chemical stability and many effects have been improved compared with vitamin E, which can not only make up for the shortcomings of vitamin E application but also broaden the application field of vitamin E. At present, in developed countries such as Europe, America, and Japan, vitamin E succinate is widely used in health foods, and due to its good water solubility and stability, the vitamin E added to most nutritional supplements (tablets and hard capsules) is vitamin E succinate. At the same time, vitamin E succinate used in the food and pharmaceutical industries is mainly catalyzed by enzymatic catalysis. In this paper, lipase (CRL) was studied. Chemical modification and immobilization were used to improve the enzymatic properties of CRL, and immobilized lipase with high stability and high activity was obtained. It was applied to the enzymatic synthesis of vitamin E succinate, and the reaction conditions were optimized to improve the yield and reduce the production cost. The review covered the research progress of the methods for enhancing the catalytic performance of immobilized enzymes and discussed its application in the synthesis of vitamin E succinate, providing new ideas and technical support for the catalytic performance enhancement of immobilized enzymes and its application in the synthesis of vitamin E succinate and promoting the production and application of vitamin E succinate.
维生素E琥珀酸酯是一种较为成熟的维生素E衍生物，与维生素E相比，其化学稳定性及多种功效均有所提高，不仅能弥补维生素E应用中的不足，还拓宽了维生素E的应用领域。目前，在欧美、日本等发达国家，维生素E琥珀酸酯广泛应用于保健食品中，且由于其良好的水溶性和稳定性，大多数营养补充剂（片剂和硬胶囊）中添加的维生素E均为维生素E琥珀酸酯。同时，食品和制药工业中使用的维生素E琥珀酸酯主要通过酶催化合成。本文以脂肪酶（CRL）为研究对象，采用化学修饰和固定化方法改善CRL的酶学性质，获得了稳定性高、活性高的固定化脂肪酶，并将其应用于维生素E琥珀酸酯的酶促合成，优化反应条件以提高产率、降低生产成本。该综述涵盖了提高固定化酶催化性能方法的研究进展，并探讨了其在维生素E琥珀酸酯合成中的应用，为提高固定化酶的催化性能及其在维生素E琥珀酸酯合成中的应用提供了新思路和技术支持，推动了维生素E琥珀酸酯的生产与应用。
