Wen Jiaqi, Pi Nana, He Fengting, Zeng Yuhao, Weng Qunfang, Luo Jianjun
College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, Guangzhou 510642, China.
Microorganisms. 2025 Mar 1;13(3):560. doi: 10.3390/microorganisms13030560.
is an effective biocontrol bacterium, with its microbial pesticides showing promise in biological control. In this study, we optimized the medium and conditions for fermenting strain F0b, developed a wettable powder formulation, and assessed its efficacy against . We screened carriers, wetting agents, dispersants, and UV protectants compatible with F0b, determining the optimal ratio and dosage. The best medium for F0b fermentation included rice flour (3.472%), ammonium chloride (4.898%), and disodium phosphate (1.871%). The ideal fermentation conditions were a 20% inoculum volume, 40 °C temperature, 80% sterile water, and a 72 h fermentation time, yielding a viable count of 1.33 × 10 CFU/mL. The final formulation contained 54.7% dried powder, 27.3% kaolinite carrier, 16% wetting agent (3:7 ratio of sodium dodecyl sulfate to sodium lignin sulfonate), and 2% ascorbic acid as a UV protectant. All quality indicators met national standards, with a viable bacteria concentration of 7 billion CFU/g. Field trials showed that the F0b wettable powder effectively controlled , with a disease index significantly lower than the control group. Control efficacy ranged from 50.58% to 73.14% over 7 to 14 days, demonstrating the commercial potential of this formulation.
是一种有效的生防细菌，其微生物农药在生物防治方面显示出前景。在本研究中，我们优化了菌株F0b的发酵培养基和条件，开发了一种可湿性粉剂配方，并评估了其对……的防治效果。我们筛选了与F0b兼容的载体、湿润剂、分散剂和紫外线保护剂，确定了最佳比例和用量。F0b发酵的最佳培养基包括米粉（3.472%）、氯化铵（4.898%）和磷酸二氢钠（1.871%）。理想的发酵条件是接种量20%、温度40℃、无菌水80%、发酵时间72小时，活菌数为1.33×10CFU/mL。最终配方包含54.7%的干粉、27.3%的高岭土载体、16%的湿润剂（十二烷基硫酸钠与木质素磺酸钠比例为3:7）和2%的抗坏血酸作为紫外线保护剂。所有质量指标均符合国家标准，活菌浓度为70亿CFU/g。田间试验表明，F0b可湿性粉剂能有效防治……，病情指数显著低于对照组。在7至1十四天内防治效果为50.58%至73.14%，证明了该配方的商业潜力。 （注：原文中部分地方表述似乎不完整，比如“against.”“controlled ”等后面应该还有具体内容，但按照要求未添加猜测内容进行完整翻译）
