Cenik Ismail, Van Slambrouck Jan, Barbarossa Annalisa, Jin Xin, Provoost An-Lies, Patel Pratik, Churchill Lucas, Bulka Ben, Haney John, Ceulemans Laurens J
Department of Thoracic Surgery, University Hospitals Leuven, 3000 Leuven, Belgium.
Laboratory of Respiratory Diseases and Thoracic Surgery (BREATHE), Department of Chronic Diseases and Metabolism, KU Leuven, 3000 Leuven, Belgium.
J Clin Med. 2025 Mar 20;14(6):2127. doi: 10.3390/jcm14062127.
: Static ice storage (SIS) has long been accepted as the conventional lung preservation method, assuming to maintain 4 °C temperature. Although never directly confirmed by temperature measurements, this assumption has been widely accepted. We aimed to measure lung and preservation temperature with SIS in porcine experiments and clinical observations. : Porcine lungs were preserved with SIS for 8 h (group I, = 3) and 3 h followed by 10 °C storage (group II, = 3). Tissue (tissueT°), first bag preservation solution (psT°) and second bag saline (salineT°) temperature were monitored. In clinical lungs ( = 4), psT° was monitored during SIS. Surface (surfaceT°) and core (coreT°) temperature were assessed before and after SIS ( = 62). : After 4 h in porcine lung group I, average tissueT° was 0.66 °C, psT° was 0.12 °C and salineT° was -0.02 °C. After 3 h in group II, average tissueT° was 1.90 °C, psT° was 0.57 °C and salineT° was 2.33 °C. In clinical observations, psT° was below 4 °C after 9-13 min and 0 °C after 78-267 min. After SIS, median surfaceT° was 1.25 °C (min-max; -3.2-9.2 °C) and coreT° was 1.45 °C (-0.4-4.8 °C). : SIS leads to rapid temperature drops below 4 °C, approaching 0 °C within 2-4 h. The post-SIS lung temperature shows considerable variability and does not consistently remain at the commonly assumed 4 °C, posing potential freezing injury to donor lungs.
静态冰储存（SIS）长期以来一直被视为传统的肺保存方法，假定能维持4°C的温度。尽管从未通过温度测量直接证实，但这一假设已被广泛接受。我们旨在通过猪实验和临床观察来测量采用SIS时肺及保存温度。：用SIS对猪肺进行8小时保存（第一组，n = 3），以及3小时后在10°C下保存（第二组，n = 3）。监测组织（组织温度T°）、第一袋保存液（psT°）和第二袋生理盐水（盐水温度T°）的温度。在临床肺脏（n = 4）中，SIS期间监测psT°。在SIS前后评估表面温度（表面温度T°）和核心温度（核心温度T°）（n = 62）。：在猪肺第一组4小时后，平均组织温度T°为0.66°C，psT°为0.12°C，盐水温度T°为 -0.02°C。在第二组3小时后，平均组织温度T°为1.90°C，psT°为0.57°C，盐水温度T°为2.33°C。在临床观察中，9 - 13分钟后psT°低于4°C，78 - 267分钟后为0°C。SIS后，表面温度T°中位数为1.25°C（最小值 - 最大值；-3.2 - 9.2°C），核心温度T°为1.45°C（-0.4 - 4.8°C）。：SIS导致温度迅速降至4°C以下，在2 - 4小时内接近0°C。SIS后的肺温度显示出相当大的变异性，并非始终保持在通常假定的4°C，这对供体肺构成潜在的冷冻损伤。
