Vizzi Esmeralda, Rosales Rita E, Piñeros Oscar, Fernández Rixio, Inaty David, López Karolina, Peña Laura, De Freitas-Linares Angela, Navarro Dianora, Neri Sandra, Durán Osmary, Liprandi Ferdinando
Laboratorio de Biología de Virus, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas 1020-A, Venezuela.
Departamento de Pediatría, Clínica Las Ciencias, Caracas 1040, Venezuela.
Viruses. 2025 Mar 13;17(3):410. doi: 10.3390/v17030410.
is the leading cause of acute gastroenteritis worldwide in young humans and animals. In 2023-2024, a relatively high rotavirus detection rate (34.5%) was detected in children with diarrhea in Caracas. All rotavirus strains were typed as P[8], using a multiplex RT-PCR assay, while the G-type was not identified. This unusual pattern, not previously observed in Venezuela, prompted the VP7 gene sequencing of nineteen strains, which displayed a high sequence identity (99.3-100%) compatible with the G3 genotype. These strains clustered into a well-supported lineage IX encompassing human reassortants of equine-like G3P[8] strains described elsewhere, showing a very close genetic relationship (99.0-99.9%). Old G3 rotavirus isolates obtained from diarrheic samples in the past were included in the analysis and grouped into lineage I together with ancestral reference G3 strains. The novel G3P[8]s carry amino acid changes in VP7-neutralizing epitopes, compared with the RotaTeq-WI78-8-vaccine strain. Full genome sequencing of a representative strain revealed a genotype constellation including an equine-like G3P[8] in a DS-1-like backbone (I2-R2-C2-M2-A2-N2-T2-E2-H2), confirming the role of animal strains as a source of diversification, and the importance of unceasingly revising molecular typing strategies and vaccine efficacy to guarantee their success.
轮状病毒是全球范围内导致人类和动物急性肠胃炎的主要病因。在2023 - 2024年期间，加拉加斯腹泻儿童中检测到相对较高的轮状病毒检出率（34.5%）。使用多重逆转录聚合酶链反应（RT-PCR）检测法，所有轮状病毒株均被分型为P[8]，而G型未被鉴定。这种在委内瑞拉以前未观察到的不寻常模式，促使对19株病毒进行VP7基因测序，结果显示其序列同一性较高（99.3 - 100%），与G3基因型相符。这些毒株聚集形成了一个支持度良好的谱系IX，其中包括在其他地方描述的马样G3P[8]毒株的人重组体，显示出非常密切的遗传关系（99.0 - 99.9%）。过去从腹泻样本中获得的旧G3轮状病毒分离株被纳入分析，并与祖先参考G3毒株一起归为谱系I。与RotaTeq-WI78-8疫苗株相比，新型G3P[8]毒株在VP7中和表位上携带氨基酸变化。对一株代表性毒株进行全基因组测序，揭示了一个基因型组合，包括在DS-1样骨架（I2-R2-C2-M2-A2-N2-T2-E2-H2）中的马样G3P[8]，证实了动物毒株作为多样化来源的作用，以及不断修订分子分型策略和疫苗效力以确保其成功的重要性。
