Proctor K G, Busija D W
Am J Physiol. 1985 Jul;249(1 Pt 2):H34-41. doi: 10.1152/ajpheart.1985.249.1.H34.
The relationship between microvessel and tissue blood flow (BF) was determined with two different techniques during changes in local vasomotor tone in the rat cremaster muscle. Whole organ and regional BF were measured with the radioactive microsphere technique (BFms) and compared with values calculated in individual arterioles (BFc) using the dual-slit cross-correlation technique. In the muscle prepared for microcirculatory observation (i.e., dissected, surgically divided into a flattened sheet, and covered with clear plastic), resting BFms was 43 +/- 3 ml X min-1 X 100 g-1, which was significantly higher than paired BFms in the contralateral undisturbed muscle (24 +/- 7 ml X min-1 X 100 g-1). Over a range in vasomotor tone, regional BFms to the edge of the tissue, which was exposed to the trauma of the surgery, was 56 +/- 7 ml X min-1 X 100 g-1 compared with 38 +/- 5 in the less traumatized center region, a significant difference of 79 +/- 31%. There was no linear relationship between arteriolar BFc and BFms. The correlation was not improved if the factors of vessel size, vasomotor tone, animal size, or muscle size were considered. Changes in arteriolar BFc (y) overestimated changes in total tissue BFms (x) by a factor of 2 (y = 2.01x - 0.6; r = 0.86), but changes in arteriolar BFc were proportional to changes in BFms if only the center region (x) of the tissue was considered (y = 1.08x - 0.1; r = 0.84). The general implication from these results is that factors that influence perfusion heterogeneity, such as surgical trauma, should be carefully considered when correlating macro- and microcirculatory measurements of BF.
在大鼠提睾肌局部血管舒缩张力变化过程中，运用两种不同技术测定微血管与组织血流量（BF）之间的关系。采用放射性微球技术（BFms）测量全器官和局部血流量，并与使用双缝互相关技术计算的单个小动脉血流量（BFc）值进行比较。在制备用于微循环观察的肌肉中（即进行解剖、手术分成扁平薄片并用透明塑料覆盖），静息状态下的BFms为43±3 ml·min⁻¹·100 g⁻¹，显著高于对侧未受干扰肌肉中的配对BFms（24±7 ml·min⁻¹·100 g⁻¹）。在一定范围的血管舒缩张力下，暴露于手术创伤的组织边缘区域的局部BFms为56±7 ml·min⁻¹·100 g⁻¹，而创伤较小的中心区域为38±5 ml·min⁻¹·100 g⁻¹，两者差异显著，达79±31%。小动脉BFc与BFms之间不存在线性关系。考虑血管大小、血管舒缩张力、动物大小或肌肉大小等因素后，相关性并未改善。小动脉BFc的变化（y）高估了总组织BFms的变化（x）两倍（y = 2.01x - 0.6；r = 0.86），但如果仅考虑组织的中心区域（x），小动脉BFc的变化与BFms的变化成比例（y = 1.08x - 0.1；r = 0.84）。这些结果的总体启示是，在关联BF的宏观和微循环测量时，应仔细考虑影响灌注异质性的因素，如手术创伤。
