Dreiner Maren, Godonou Elie-Tino, Mündermann Annegret, Tascilar Koray, Schett Georg, Zaucke Frank, Liphardt Anna-Maria, Niehoff Anja
Institute of Biomechanics and Orthopaedics, German Sport University Cologne, Köln, Germany.
Department of Internal Medicine - Rheumatology & Immunology, Universitätsklinikum Erlangen & Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
Osteoarthr Cartil Open. 2025 Feb 28;7(2):100597. doi: 10.1016/j.ocarto.2025.100597. eCollection 2025 Jun.
To investigate the effects of 21 days bed rest immobilization (with and without exercise and nutrition intervention) on serum concentrations of cartilage homeostasis biomarkers in healthy individuals.
Twelve male volunteers (age 34.2 ± 8.3 years; BMI 22.4 ± 1.7 kg/m) participated in 6 days of baseline data collection (BDC), 21 days of 6° head-down-tilt (HDT) bed rest (CON) + interventions HDT + resistive vibration exercise (RVE; 2 times/week; 25 min) and HDT + RVE + nutrition (NeX; 0.6 g/kg body weight/day whey protein and 90 mmol KHCO/day bicarbonate supplementation), and 6 days of recovery (R) in a cross-over designed study. The starting HDT condition was randomized (CON-RVE-NeX, RVE-NeX-CON, NeX-CON-RVE). Blood samples were collected before, during and after HDT. Serum concentrations of COMP, MMP-3, MMP-9, YKL-40 and resistin were analyzed.
The main effect of time was significant for all biomarkers tested (p < 0.001). While COMP (-36 % at HDT5, p < 0.001) and MMP-3 (-36 % at HDT21, p < 0.001) decreased during HDT bed rest, MMP-9 (+18 % at HDT5, p < 0.001) and resistin (+13 % at HDT21, p < 0.001) increased during HDT bed rest. Interestingly, during recovery, YKL-40 levels increased (+13 % at R1, p = 0.022), while MMP-9 levels decreased (-19 % at R6, p = 0.035). We identified correlations between COMP and MMP-3 (r = 0.58, p < 0.001) as well as between MMP-9 and resistin (r = 0.58, p < 0.001).
Immobilization affects serum concentrations of cartilage homeostasis biomarkers suggesting changes in cartilage metabolism that do not completely recover during re-ambulation. Both interventions had only minimal effects.
研究21天卧床制动（有无运动和营养干预）对健康个体血清中软骨稳态生物标志物浓度的影响。
12名男性志愿者（年龄34.2±8.3岁；体重指数22.4±1.7kg/m²）参与了一项交叉设计研究，包括6天的基线数据收集（BDC）、21天的6°头低位倾斜（HDT）卧床休息（CON）+干预措施HDT+阻力振动运动（RVE；每周2次；25分钟）和HDT+RVE+营养（NeX；0.6g/kg体重/天的乳清蛋白和90mmol KHCO₃/天的碳酸氢盐补充），以及6天的恢复（R）期。起始HDT条件随机分配（CON-RVE-NeX、RVE-NeX-CON、NeX-CON-RVE）。在HDT之前、期间和之后采集血样。分析血清中COMP、MMP-3、MMP-9、YKL-40和抵抗素的浓度。
所检测的所有生物标志物的时间主效应均显著（p<0.001）。在HDT卧床休息期间，COMP（HDT5时下降36%，p<0.001）和MMP-3（HDT21时下降36%，p<0.001）降低，而MMP-9（HDT5时升高18%，p<0.001）和抵抗素（HDT21时升高13%，p<0.001）升高。有趣的是，在恢复期间，YKL-40水平升高（R1时升高13%，p=0.022），而MMP-9水平降低（R6时降低19%，p=0.035）。我们确定了COMP与MMP-3之间（r=0.58，p<0.001）以及MMP-9与抵抗素之间（r=0.58，p<0.001）的相关性。
制动会影响血清中软骨稳态生物标志物的浓度，提示软骨代谢发生变化，且在重新活动期间不会完全恢复。两种干预措施的影响都很小。
