Horiguchi Yusuke, Wu Haiyun, Murata Masataka, Matsumoto Haruto, Ohnuki Hitoshi, Endo Hideaki
Department of Ocean Sciences, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato-ku, Tokyo, 108-8477, Japan.
Hokkaido Industrial Technology Center, 379-32, Kikyocho, Hakodate-shi, Hokkaido, 041-0801, Japan.
Fish Physiol Biochem. 2025 Mar 27;51(2):74. doi: 10.1007/s10695-025-01488-w.
In our laboratory, we have been actively investigating the physiological responses of free-swimming Nile tilapia (Oreochromis niloticus) to acute stress. One of the crucial indicators we monitored was the fluctuation in fish blood glucose levels. To achieve this, we have implemented a wireless biosensor system designed to measure glucose concentration within the fish eyeball interstitial sclera fluid. In this study, in addition to glucose concentration, we have incorporated a triaxial acceleration sensor onto the fish to track its movements and acceleration patterns. This multi-faceted approach enables us to simultaneously assess the stress response and physical activity of the fish under various stressors. Our study specifically focuses on stressors such as ammonia exposure and social interactions among individuals. Remarkably, our findings have revealed intriguing insights into the fish's stress response. While the biosensor consistently recorded an increase in glucose levels in response to all stressors, the triaxial acceleration sensor data exhibited distinct patterns of behavior during each stressor application. This variance in acceleration data suggests that the fish's response to different stressors is not uniform and can be differentiated through their movement patterns. This novel and integrated approach, merging biosensor technology with triaxial acceleration measurements, holds immense promise in shedding light on the nuanced intricacies of fish physiology and biochemistry. It opens new vistas for comprehending how these aquatic creatures cope with acute stressors and adapt to their ever-changing environments, ultimately contributing to the broader body of knowledge in the field of fish physiology and biochemistry.
在我们实验室，我们一直在积极研究自由游动的尼罗罗非鱼（Oreochromis niloticus）对急性应激的生理反应。我们监测的关键指标之一是鱼血糖水平的波动。为实现这一点，我们实施了一个无线生物传感器系统，旨在测量鱼眼球间质巩膜液中的葡萄糖浓度。在本研究中，除了葡萄糖浓度外，我们还在鱼身上安装了一个三轴加速度传感器，以追踪其运动和加速度模式。这种多方面的方法使我们能够同时评估鱼在各种应激源下的应激反应和身体活动。我们的研究特别关注诸如氨暴露和个体间社会互动等应激源。值得注意的是，我们的研究结果揭示了关于鱼应激反应的有趣见解。虽然生物传感器始终记录到所有应激源导致的葡萄糖水平升高，但三轴加速度传感器数据在每次施加应激源时都呈现出不同的行为模式。加速度数据的这种差异表明，鱼对不同应激源的反应并不一致，可以通过它们的运动模式加以区分。这种将生物传感器技术与三轴加速度测量相结合的新颖综合方法，在揭示鱼类生理和生物化学的细微复杂之处方面具有巨大潜力。它为理解这些水生生物如何应对急性应激源并适应不断变化的环境开辟了新视野，最终为鱼类生理和生物化学领域的更广泛知识体系做出贡献。
