Gas chromatography-mass spectroscopy analysis of juvenile hormone released by insect corpora allata.

Corpora allata incubated in appropriate medium release several compounds including juvenile hormones. Juvenile hormones (14C labeled or unlabeled) were extracted with hexane and directly analyzed by gas chromatography-mass spectroscopy. This method allowed the qualitative and quantitative analysis of total released juvenile hormone. It could also be used as a routine assay for evaluation of corpus allatum activity. Data obtained by this method were compared to those obtained by radiochemical assay.