Ravichandran Subash Kannan, Silvaster Blessy, Rajendran Selvakumar, Wong Jonathan W C, Ganesan Brindha, Johnravindar Davidraj
Sustainable Environment and Bioresource Technology Laboratory, Department of Nanobiotechnology, PSG Institute of Advanced Studies, Coimbatore, India.
Nanobiotechnology Laboratory, Department of Nanobiotechnology, PSG Institute of Advanced Studies, Coimbatore, India.
Environ Technol. 2025 Aug;46(19):3887-3903. doi: 10.1080/09593330.2025.2480319. Epub 2025 Mar 27.
Hydrogen gas (H) is a promising renewable resource, and its production from organic waste has gained significant attention as an alternative to traditional energy sources. This study investigates hydrogen production from cassava industry waste residue (CWR) by incorporating hydrochar-supported nanoparticles (HSNPs) containing nickel (Ni) and iron (Fe) nanoparticles (NPs). Ni and FeO NPs were synthesised through the hydrothermal carbonisation (HTC) method and analyzed for their impact on biohydrogen production. The results indicated a maximum volumetric hydrogen production rate of 1013 mL/gCWR, with a substrate degradation efficiency of 80%. Addition of 0.5 mol Ni-FeO hydrochar was found to be optimal which enhanced the overall cumulative hydrogen production by 41.015%, and higher COD removal efficiency by 90% was obtained as compared to hydrochar without additives. The toxicity of NPs was assessed through bacterial viability, which examined various loading concentrations of NPs in a glucose medium. The findings revealed that supplementing hydrochar-supported NiO and FeO NPs within an optimal range for CWR can significantly improve hydrogen productivity. Additionally, HSNPs can enhance hydrogenase activity and electron transfer efficiency, which are beneficial to bio-H evolution. However, excessive HSNP addition may be toxic to microbes and further inhibit H production. This study presents an effective method for promoting the evolution rate of H gas.
氢气（H₂）是一种很有前景的可再生资源，利用有机废弃物生产氢气作为传统能源的替代方式已受到广泛关注。本研究通过加入负载有含镍（Ni）和铁（Fe）纳米颗粒（NPs）的水热炭（HSNPs），研究了木薯工业废渣（CWR）制氢情况。通过水热碳化（HTC）法合成了Ni和FeO NPs，并分析了它们对生物制氢的影响。结果表明，最大体积产氢速率为1013 mL/gCWR，底物降解效率为80%。发现添加0.5 mol Ni-FeO水热炭是最佳的，这使总累积产氢量提高了41.015%，与无添加剂的水热炭相比，化学需氧量（COD）去除效率更高，达到了90%。通过细菌活力评估了NPs的毒性，该评估检测了葡萄糖培养基中不同负载浓度的NPs。研究结果表明，在CWR的最佳范围内添加负载NiO和FeO的水热炭可显著提高产氢率。此外，HSNPs可提高氢化酶活性和电子转移效率，这有利于生物产氢。然而，过量添加HSNP可能对微生物有毒，并进一步抑制氢气产生。本研究提出了一种提高氢气析出速率的有效方法。
