Kraus R J, Foster S J, Ganther H E
Anal Biochem. 1985 Jun;147(2):432-6. doi: 10.1016/0003-2697(85)90293-3.
A rapid and simple method for the separation of trimethylselenonium ion and other cationic forms of selenium in urine by HPLC on a strong cation exchanger is described. Most of the inorganic salts in urine are removed prior to chromatography by means of ethanolic precipitation, thus minimizing interferences. Following sample loading and elution with 0.003 M ammonium phosphate (pH 4), a linear gradient to 0.33 M ammonium phosphate (pH 4) is employed. Complete separation of the trimethylselenonium ion from four other selenonium compounds was achieved, and good recovery of the compounds was obtained for the desalting and chromatographic procedures. The procedure was successfully employed to demonstrate that dimethylselenocysteineselenonium iodide and Se-methylselenomethionineselenonium iodide are extensively metabolized when administered to rats, and that trimethylselenonium ion is a major urinary metabolite of both compounds.
本文描述了一种在强阳离子交换剂上通过高效液相色谱法快速、简单地分离尿中三甲基硒离子和其他阳离子形式硒的方法。尿中的大多数无机盐在色谱分析前通过乙醇沉淀法去除，从而将干扰降至最低。进样后，用0.003M磷酸铵（pH 4）洗脱，然后采用线性梯度至0.33M磷酸铵（pH 4）。实现了三甲基硒离子与其他四种硒鎓化合物的完全分离，并且在脱盐和色谱分析过程中这些化合物的回收率良好。该方法成功地用于证明，给大鼠施用二甲基硒代半胱氨酸硒碘化物和硒甲基硒代蛋氨酸硒碘化物后，它们会被广泛代谢，并且三甲基硒离子是这两种化合物的主要尿代谢产物。
