Yuce Merve, Aydin Murat, Turan Metin, Ilhan Emre, Ekinci Melek, Agar Guleray, Yildirim Ertan
Atatürk University, Faculty of Agriculture, Department of Horticulture, Erzurum, Turkey.
Atatürk University, Faculty of Agriculture, Department of Agricultural Biotechnology, Erzurum, Turkey.
Plant Physiol Biochem. 2025 Jun;223:109798. doi: 10.1016/j.plaphy.2025.109798. Epub 2025 Mar 20.
Salinity is one of the most important problems that threaten agricultural production, especially in arid and semiarid areas. Strigolactones (SLs) are important in providing tolerance to various abiotic stresses in plants. The study was carried out in a hydroponic system to determine the effects of external GR24 (were applied as a foliar spray; 0, 10, and 20 μM) applications at different doses on plant growth and some physiological, biochemical, and gene expression in two pepper genotype (Yalova and Maraş) grown under salt stress (0 and 100 mM NaCl). Plants were harvested and measured 10 days after the NaCl treatments. At the end of the research, it was determined that salt stress negatively affected plant growth in both genotype. Still, SL applications positively affected plant development both under normal and salt stress. While salt stress increased the amount of hydrogen peroxide (HO) and malondialdehyde (MDA), SL application caused a decrease in these parameters. Salt stress negatively affected the amount of chlorophyll and photosynthetic properties in both genotype, whereas SL applications mitigated this negative effect. SL applications caused a significant increase in antioxidant enzyme activities under both normal and salt stress conditions. SL content, which decreased with salt stress, increased with exogenous SL application. The content of other plant nutrients except sodium (Na) and chloride (Cl) decreased significantly in pepper seedlings grown under salt stress. External SL applications increased the uptake of these nutrients, especially under salt stress. In addition, the expression levels of CIPK3, CBL2, CCD7, DMAX2, PsbA, PsbB, PsbP1, TIP1;2, TIP5;1, SOS1, SOS2 and HKT2;2 genes were investigated in this study. It was observed that the expression levels of CCD7, DMAX2, SOS1, SOS2, and HKT2;2 genes increased with salinity stress, especially in the Maraş genotype, while SL applications decreased these expression levels. In the study, it was determined that especially exogenous 20 μM SL application could significantly reduce the negative effects of salt stress in pepper.
盐度是威胁农业生产的最重要问题之一，尤其是在干旱和半干旱地区。独脚金内酯（SLs）在植物对各种非生物胁迫的耐受性方面起着重要作用。本研究在水培系统中进行，以确定不同剂量的外源GR24（以叶面喷施的方式施用；0、10和20 μM）对在盐胁迫（0和100 mM NaCl）下生长的两种辣椒基因型（亚洛瓦和马拉什）的植物生长以及一些生理、生化和基因表达的影响。在NaCl处理10天后收获并测量植株。研究结束时，确定盐胁迫对两种基因型的植物生长均有负面影响。然而，SL处理在正常和盐胁迫条件下均对植物发育有积极影响。盐胁迫增加了过氧化氢（HO）和丙二醛（MDA）的含量，而SL处理使这些参数降低。盐胁迫对两种基因型的叶绿素含量和光合特性均有负面影响，而SL处理减轻了这种负面影响。在正常和盐胁迫条件下，SL处理均导致抗氧化酶活性显著增加。随着盐胁迫而降低的SL含量，随着外源SL的施用而增加。在盐胁迫下生长的辣椒幼苗中，除钠（Na）和氯（Cl）外的其他植物养分含量显著降低。外源SL处理增加了这些养分的吸收，尤其是在盐胁迫下。此外，本研究还调查了CIPK3、CBL2、CCD7、DMAX2、PsbA、PsbB、PsbP1、TIP1;2、TIP5;1、SOS1、SOS2和HKT2;2基因的表达水平。观察到CCD7、DMAX2、SOS1、SOS2和HKT2;2基因的表达水平随着盐胁迫而增加，尤其是在马拉什基因型中，而SL处理降低了这些表达水平。在该研究中，确定特别是外源20 μM SL处理可显著降低盐胁迫对辣椒的负面影响。
