Zhao Yingying, Ma Qiurui, Chen Hong, Zhao Shangyi, Liang Xuexin, Dang Dan
College of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450001, China.
College of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450001, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Sep 5;337:126101. doi: 10.1016/j.saa.2025.126101. Epub 2025 Mar 22.
Optimal levels of Cu are essential for the proper functioning of organisms, while excessive quantities lead to significant detrimental effects. In this study, a Schiff base fluorescent probe XSF based on 3-aminobenzo[b]thiophene-2-carboxylate was been successfully designed and synthesized. The synthesis pathway of XSF is highly convenient, enabling the detection of Cu through a dual channel mechanism involving fluorescence quenching and colorimetric response. It exhibits exceptional sensitivity with an extremely low detection limit (2.91 × 10 M), rapid response kinetics (less than 10 s), and applicability within a pH range of 3-9. The fluorescence quenching of XSF by Cu was reasonably explained using techniques such as H NMR and DFT calculations. In addition, satisfactory recovery rates were achieved in the detection of Cu in actual samples such as drinking water, ginger, and chocolate. The visual detection method was successfully applied to real samples including cellophane noodles and towels, and XSF test papers were prepared, fully demonstrating the potential of XSF for practical testing applications. The XSF-Cu system demonstrates high sensitivity in the detection of GSH (LOD = 8.73 × 10 M) and exhibits a remarkable enhancement in fluorescence response. XSF has successfully demonstrated its application in the detection of Cu and GSH within cells, thus highlighting its potential as a promising tool in the field of biology.
最佳水平的铜对于生物体的正常功能至关重要，而过量的铜会导致显著的有害影响。在本研究中，基于3-氨基苯并[b]噻吩-2-羧酸酯成功设计并合成了一种席夫碱荧光探针XSF。XSF的合成途径非常简便，能够通过荧光猝灭和比色响应的双通道机制检测铜。它具有出色的灵敏度，检测限极低（2.91×10⁻⁸ M），响应动力学快速（小于10秒），并且在pH值为3至9的范围内适用。利用¹H NMR和DFT计算等技术合理解释了铜对XSF的荧光猝灭。此外，在饮用水、生姜和巧克力等实际样品中检测铜时，回收率令人满意。视觉检测方法成功应用于包括粉丝和毛巾在内的实际样品，并制备了XSF试纸，充分证明了XSF在实际检测应用中的潜力。XSF-Cu系统在检测谷胱甘肽（LOD = 8.73×10⁻⁷ M）时表现出高灵敏度，并在荧光响应方面有显著增强。XSF已成功证明其在细胞内检测铜和谷胱甘肽的应用，从而突出了其作为生物学领域有前途工具的潜力。
