Gu Wenfei, Ma Xiaoxue, Yang Chuanyan, Jiang Dongli, Fan Hongmei, Wang Lingling, Song Linsheng
Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian 116023, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian 116023, China.
Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian 116023, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian 116023, China.
Comp Biochem Physiol B Biochem Mol Biol. 2025 Jun-Jul;278:111092. doi: 10.1016/j.cbpb.2025.111092. Epub 2025 Mar 25.
Inositol 1,4,5-trisphosphate receptor 2 (IPR2) is an essential Ca release channel protein located in the endoplasmic reticulum (ER), and plays a significant role in responding to various environmental stimuli. In the present study, the function of IPR2 from Yesso scallop Patinopecten yessoensis (PyIPR2) in regulating the Ca-mediated unfolded protein response (UPR) and apoptosis after high temperature (25 °C) treatment was investigated. Three MIR domains, one RYDR_ITPR domain, one RIH_assoc domain and one Ion_trans domain were identified in PyIPR2. Both D-myo-inositol-1,4,5-triphosphate (IP3, an activator of IPR) and high temperature significantly upregulated the mRNA expression level of PyIPR2 and genes related to apoptosis and the UPR, and also increased intracellular Ca content (p < 0.05). Furthermore, the IPR antagonist 2-aminoethyl diphenylborinate (2-APB) had the opposite effect, decreasing intracellular Ca content and the mRNA expression level of PyIPR2, glucose regulated protein 78 (PyGRP78) and PyCaspase-3 (p < 0.05). However, the apoptosis rate and Caspase-3 activity remained comparable to those in the injection control group. These findings indicate that PyIPR2 mediates UPR and apoptosis in scallop haemocytes by regulating Cacontent and distribution, and providing insight into the cellular responses of scallops to high temperature.
肌醇-1,4,5-三磷酸受体2（IPR2）是一种位于内质网（ER）的重要钙离子释放通道蛋白，在响应各种环境刺激中发挥重要作用。在本研究中，研究了栉孔扇贝（Patinopecten yessoensis）的IPR2（PyIPR2）在高温（25°C）处理后调节钙离子介导的未折叠蛋白反应（UPR）和细胞凋亡中的功能。在PyIPR2中鉴定出三个MIR结构域、一个RYDR_ITPR结构域、一个RIH_assoc结构域和一个Ion_trans结构域。D-肌醇-1,4,5-三磷酸（IP3，IPR的激活剂）和高温均显著上调了PyIPR2以及与细胞凋亡和UPR相关基因的mRNA表达水平，同时也增加了细胞内钙离子含量（p<0.05）。此外，IPR拮抗剂2-氨基乙基二苯基硼酸盐（2-APB）具有相反的作用，降低了细胞内钙离子含量以及PyIPR2、葡萄糖调节蛋白78（PyGRP78）和PyCaspase-3的mRNA表达水平（p<0.05）。然而，凋亡率和Caspase-3活性与注射对照组相当。这些发现表明，PyIPR2通过调节钙离子含量和分布介导栉孔扇贝血细胞中的UPR和细胞凋亡，为深入了解栉孔扇贝对高温的细胞反应提供了见解。
