Rahimian Mohammadreza, Deyhim Hanieh, Shirazi-Zavaragh Samaneh, Zeynali Majid, Bonabi Esat, Zarghami Nosratollah
Department of Clinical Biochemistry and Laboratory Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Medical Microbiology, Faculty of Medicine, Istanbul Aydin University, Istanbul, Turkey.
Department of Clinical Biochemistry and Laboratory Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Microb Pathog. 2025 Jun;203:107506. doi: 10.1016/j.micpath.2025.107506. Epub 2025 Mar 25.
Colibacillosis, brought on by Avian Pathogenic Escherichia coli (APEC), is a significant risk to poultry farming. The management of colibacillosis is made more difficult by the increasing levels of antimicrobial resistance (AMR) in APEC isolates. The antibiotic resistance profiles, antibiotic resistance gene (ARG) presence, and phage susceptibility of 130 APEC isolates were examined in this investigation. The highest resistance against antibiotics was detected against tetracycline and nalidixic acid, with 86.15% and 85.38%, respectively, among isolates. A high frequency of ARGs was found in the data, especially the positively correlated bla (95.38%) and tetA (85.38%) genes. The 20 bacteriophages we recovered were further described, with PhEcoAP90 having the widest host range. A 14 ± 1.41 nm tail and a 52.20 ± 2.94 nm head were revealed by TEM examination. A one-step growth experiment observed a 20-min latent period and a 58 PFU/cell burst size. PhEcoAP90 showed instability at high temperatures and extreme pH values. These results imply that although PhEcoAP90 exhibits potential as an antibiotic alternative for managing APEC infections, its use in severe settings may be restricted. To help combat AMR in chicken farming, we also suggest a unique AMR surveillance system that includes phage sensitivity, ARG prevalence, and antibiotic resistance profiles. In conclusion, our research emphasizes the need for integrated AMR surveillance systems in the poultry industry and the promise of phages as efficient alternative therapeutic approaches in regulating AMR in APEC.
禽致病性大肠杆菌（APEC）引发的大肠杆菌病对家禽养殖构成重大风险。APEC分离株中抗菌药物耐药性（AMR）水平的不断提高，使得大肠杆菌病的管理更加困难。本研究检测了130株APEC分离株的抗生素耐药谱、抗生素耐药基因（ARG）的存在情况以及噬菌体敏感性。分离株中对四环素和萘啶酸的耐药性最高，分别为86.15%和85.38%。数据中发现ARG的频率很高，尤其是呈正相关的bla（95.38%）和tetA（85.38%）基因。我们对回收的20种噬菌体进行了进一步描述，其中PhEcoAP90的宿主范围最广。透射电镜检查显示其尾部为14±1.41nm，头部为52.20±2.94nm。一步生长实验观察到潜伏期为20分钟，每细胞爆发量为58个噬菌斑形成单位（PFU）。PhEcoAP90在高温和极端pH值下表现出不稳定性。这些结果表明，尽管PhEcoAP90作为管理APEC感染的抗生素替代品具有潜力，但其在严重情况下的使用可能会受到限制。为了帮助应对养鸡业中的AMR问题，我们还建议建立一个独特的AMR监测系统，该系统包括噬菌体敏感性、ARG流行情况和抗生素耐药谱。总之，我们的研究强调了家禽行业综合AMR监测系统的必要性，以及噬菌体作为调节APEC中AMR的有效替代治疗方法的前景。
