黑鱼感染后基因家族的鉴定与表达分析
Identification and Expression Analyses of Gene Family in Snakehead () Following Infection.
作者信息
Han Xiufeng, Su Xue, Che Mingyue, Liu Lanhao, Nie Pin, Wang Su
机构信息
School of Marine Science and Engineering, Qingdao Agricultural University, 700 Greatwall Road, Qingdao 266109, China.
Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao 266237, China.
出版信息
Genes (Basel). 2025 Feb 22;16(3):253. doi: 10.3390/genes16030253.
BACKGROUND/OBJECTIVES: The interleukin 17 (IL-17) family, known for its proinflammatory properties, is important in immune responses against bacterial and fungal infections. To exert its immune function, the IL-17 family typically binds to IL-17 receptor (IL-17R) to facilitate signal transduction.
METHODS
This study identified, cloned and analyzed seven IL-17 and nine IL-17R family members in snakeheads.
RESULTS
A duplication event occurred in snakehead and , but bioinformatics analyses indicated that these genes were conserved in both protein domains and evolutionary processes. Tissue distribution analysis revealed that / were widely distributed in the detected tissues, with relatively high expression levels in immune tissues. Upon stimulation, most members were expressed, particularly , , , , , , and , which were significantly upregulated in gill and intestine.
CONCLUSIONS
These results suggested that and played a crucial role in mucosal immunity against bacterial infection, providing insights into immunoprophylactic strategies for bacterial diseases in aquaculture.
背景/目的：白细胞介素17（IL-17）家族以其促炎特性而闻名，在针对细菌和真菌感染的免疫反应中起重要作用。为发挥其免疫功能，IL-17家族通常与IL-17受体（IL-17R）结合以促进信号转导。
方法
本研究鉴定、克隆并分析了乌鳢中的7个IL-17和9个IL-17R家族成员。
结果
乌鳢的 和 发生了复制事件，但生物信息学分析表明这些基因在蛋白质结构域和进化过程中均保守。组织分布分析显示， / 在检测到的组织中广泛分布，在免疫组织中的表达水平相对较高。在 刺激后，大多数成员表达，特别是 、 、 、 、 、 和 ，它们在鳃和肠道中显著上调。
结论
这些结果表明， 和 在针对细菌感染的黏膜免疫中起关键作用，为水产养殖中细菌性疾病的免疫预防策略提供了见解。
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