不同群体的SSR遗传分子标记与形态差异研究
Research on SSR Genetic Molecular Markers and Morphological Differences of Different Populations.
作者信息
Liang Yixin, Huang Changqing, Wang Pei, Xiao Hewei, Wang Zi'ao, Zeng Jiawei, Wang Xiaoqing, Xiong Shuting, Hu Yazhou, Qin Qin
机构信息
College of Fisheries, Hunan Agricultural University, Changsha 410128, China.
College of Biology and Environmental Sciences, Jishou University, Jishou 416000, China.
出版信息
Genes (Basel). 2025 Mar 7;16(3):318. doi: 10.3390/genes16030318.
BACKGROUND/OBJECTIVES: The Chinese soft-shelled turtle () is an important species in freshwater aquaculture. Genetic admixture and degradation due to rapid industry expansion threaten sustainable development. This study aims to assess the genetic diversity and structure of six populations for better management.
METHODS
We combined morphological analysis and microsatellite markers to evaluate the genetic diversity of six populations. A discriminant function based on morphology was developed, achieving 71.4% classification accuracy. Two SSR markers were identified to specifically distinguish the HS population.
RESULTS
The six populations were classified into three subgroups. Frequent gene flow was observed among the CY, W, and DT populations, with most genetic variation occurring within individuals. However, significant genetic differentiation was detected between populations. While gene flow enhanced diversity, it suppressed differentiation.
CONCLUSIONS
This study provides insights into the genetic structure and diversity of six populations. The discriminant function and SSR markers offer a basis for germplasm conservation and management, supporting sustainable aquaculture development.
背景/目的：中华鳖是淡水养殖中的重要物种。快速的产业扩张导致的遗传混合和退化威胁着可持续发展。本研究旨在评估六个中华鳖种群的遗传多样性和结构，以实现更好的管理。
方法
我们结合形态学分析和微卫星标记来评估六个种群的遗传多样性。开发了基于形态学的判别函数，分类准确率达到71.4%。鉴定出两个SSR标记可特异性区分HS种群。
结果
六个种群被分为三个亚组。在CY、W和DT种群之间观察到频繁的基因流动，大多数遗传变异发生在个体内部。然而，种群之间检测到显著的遗传分化。虽然基因流动增加了多样性，但抑制了分化。
结论
本研究为六个中华鳖种群的遗传结构和多样性提供了见解。判别函数和SSR标记为种质保护和管理提供了基础，支持可持续水产养殖发展。
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